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Merck

MABN790

Sigma-Aldrich

Anti-Shank3 Antibody, clone 16E2.3

clone 16E2.3, from mouse

Synonym(e):

SH3 and multiple ankyrin repeat domains protein 3, Shank3, Proline-rich synapse-associated protein 2, ProSAP2, Shank3

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

16E2.3, monoclonal

Speziesreaktivität

mouse

Speziesreaktivität (Voraussage durch Homologie)

human (based on 100% sequence homology)

Methode(n)

western blot: suitable

Isotyp

IgG2aκ

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... SHANK3(85358)

Allgemeine Beschreibung

SH3 and multiple ankyrin repeat domains protein 3 (UniProt Q9BYB0; also known as Proline rich synapse associated protein 2, ProSAP2, Shank postsynaptic density protein, Shank3) is encoded by the SHANK3 (also known as DEL22q13.3, PROSAP2, PSAP2, SCZD15, SPANK-2) gene (Gene ID 85358) in human. Shank3/ProSAP2 belongs to the ProSAP/Shank family of scaffolding proteins, which are important dendritic spines postsynaptic density (PSD) complex components that play an important role in linking membrane glutamate receptors to intracellular signaling proteins and actin cytoskeleton. Shank proteins interact with adaptor proteins and assembles glutamate receptors at the postsynaptic membrane via multiple domains, including ankyrin repeat, proline-rich, SH3, PSD95/disc-large/zona occludens-1 (PDZ), and SAM domains. Shanks, together with the adaptor protein Homer and the endocytic vesicle-associated protein dynamin-3, are required for proper localization of the endocytic zone in close proximity to the PSD. Shank-3 induces formation of functional dendritic spines in aspiny cerebellar neurons, whereas Shank-3 knockdown results in reduced spine density and size in cultured hippocampal neurons. SHANK3 gene mutations are known causes of mental retardation and autism spectrum disorders.

Spezifität

Expected to react with both isoform 1 (A) and 2 (B).

Immunogen

Epitope: Internal (C-terminal half)
GST-tagged recombinant protein corresponding to the C-terminal half of human Shank3.

Anwendung

Research Category
Neurowissenschaft
Research Sub Category
Entwicklungsabhängige Signalübertragung
This Anti-Shank3 Antibody, clone 16E2.3 is validated for use in Western Blotting for the detection of Shank3.

Qualität

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Shank3 in 10 µg of mouse brain tissue lysate.

Zielbeschreibung

~185 kDa observed. Multiplet banding pattern as a result of isoforms and/or posttranslational modifications. Uncharacterized band(s) may appear in some lysates.

Physikalische Form

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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