MABF2250

Anti-NKp44 (CD336) Antibody, clone 3.43.13

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 3.43.13, monoclonal
• Application: FACS, ICC
• Citations: -

Eigenschaften

• conjugate: unconjugated
• antibody form: purified antibody
• clone: 3.43.13, monoclonal
• purified by: using protein G
• species reactivity: mouse, human
• concentration: (Please refer to lot specific datasheet.)
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable
• isotype: IgG1κ
• UniProt accession no.: O95944
• target post-translational modification: unmodified

Beschreibung

General description

30.68 kDa calculated.

NKp44-human crystallizable fragment (Fc) fusion protein.

Application

Affects Function Analysis: A representative lot activated both NK92-44wt and NK92-44mut cell lines (Brusilovsky, M., et. al. (2015). Eur J Immunol. 45(4):1180-91).


Immunocytochemistry Analysis: A representative lot detected NKp44 (CD336) in Immunocytochemistry applications (Brusilovsky, M., et. al. (2015). Eur J Immunol. 45(4):1180-91).

Flow Cytometry Analysis: A representative lot detected NKp44 (CD336) in Flow Cytometry applications Barrow, A.D., et. al. (2018). Cell. 172(3):534-548; Kikuchi-Maki, A., et. al. (2003). J Immunol. 171(7):3415-25; Fuchs, A., et. al. (2005). Blood. 106(6):2076-82; Brando, C., et. al. (2005). J Leukoc Biol. 78(2):359-71; Eisenberg, V., et. al. (2017). Front Immunol. 8:1212).

Biochem/physiol Actions

Clone 3.43.13 is a mouse monoclonal antibody that detects human Natural cytotoxicity triggering receptor 2 (NKp44) protein.

Physical form

Purified mouse monoclonal antibody IgG1 in PBS without any preservatives.

Preparation Note

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Flow Cytometry in rhIL-2 stimulated human peripheral blood mononuclear cells (PBMCs) for 7 days (100 U per mL).

Flow Cytometry Analysis: 1 µg of this antibody detected NKp44 (CD336) in rhIL-2 stimulated one million human peripheral blood mononuclear cells (PBMCs) for 7 days (100 U per mL).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.