MABF2248

Anti-EBV LMP1 Antibody, clone S12

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: S12, monoclonal
• Application: FACS, ICC, IF, IHC, IP, WB
• Citations: -

Eigenschaften

• conjugate: unconjugated
• antibody form: purified antibody
• clone: S12, monoclonal
• purified by: using protein G
• species reactivity: mouse, virus
• concentration: (Please refer to lot specific datasheet.)
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG2aκ
• UniProt accession no.: P03230
• target post-translational modification: unmodified

Beschreibung

General description

A fusion protein (pKH548) consisting of 189 amino acids from the C-terminus region of LMP1 coupled to beta-galactosidase.

~59 kDa observed; 41.98 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Application

Immunocytochemistry Analysis: A representative lot detected EBV LMP1 in Immunocytochemistry applications (Xu, J., et. al. (2002). J Virol. 76(8):4080-6).

Flow Cytometry Analysis: A representative lot detected EBV LMP1 in Flow Cytometry applications (Mann, K.P., et. al. (1985). J Virol. 55(3):710-20).

Western Blotting Analysis: A representative lot detected EBV LMP1 in Western Blotting applications (Choi, I.K., et. al. (2018). Proc Natl Acad Sci USA. 115(4):E686-E695; Young, L.S., et. al. (1988). J Gen Virol. 69 ( Pt 5):1051-65; Mann, K.P., et. al. (1985). J Virol. 55(3):710-20).

Immunofluorescence Analysis: A representative lot detected EBV LMP1 in Immunofluorescence applications (Mann, K.P., et. al. (1985). J Virol. 55(3):710-20).

Immunohistochemistry Analysis: A representative lot detected EBV LMP1 in Immunohistochemistry applications (Brink, A.A., et. al. (1997). J Clin Pathol. 50(11):911-8; Khabir, A., et. al. (2005). Virol J. 2:39).

Immunoprecipitation Analysis: A representative lot immunoprecipitated EBV LMP1 in Immunoprecipitation applications (Young, L.S., et. al. (1988). J Gen Virol. 69 ( Pt 5):1051-65).

Biochem/physiol Actions

Clone S12 is a mouse monoclonal antibody that specifically detects Latent membrane protein 1 (LMP1) in Epstein-Barr virus transformed cells.

Physical form

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in Raji cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected LMP1 in lysates from Raji cells.

Other Notes

Virus transformed human cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.