MABC580

Anti-Clathrin Heavy Chain Antibody, clone 6F10.1

clone 6F10.1, from mouse

• Synonym(e): Clathrin heavy chain 1, Clathrin Heavy Chain, Clathrin heavy chain on chromosome 17, CLH-17
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 6F10.1, monoclonal
• Application: FACS, ICC, WB
• Citations: 2

Eigenschaften

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 6F10.1, monoclonal
• species reactivity: human, mouse, rat
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_004850
• UniProt accession no.: Q00610
• shipped in: wet ice
• target post-translational modification: unmodified
• Quality Level: 100
• Gene Information: human ... CLTC(1213)

Beschreibung

General description

Clathrin heavy chain 1 (UniProt Q00610; also known as Clathrin heavy chain on chromosome 17, Clathrin heavy polypeptide (Hc), Clathrin heavy polypeptide-like 2, CLH-17) is encoded by the CLTC (also known as CHC, CLH17, CLTCL2, KIAA0034) gene in human (Entrez Gene ID 1213). Clathrin is the major protein component of the polyhedral coat of coated pits and vesicles. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of various macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.

~190 kDa observed

Immunogen

GST-tagged recombinant protein corresponding to human Clathrin Heavy Chain near the C-terminus.

Application

Anti-Clathrin Heavy Chain Antibody, clone 6F10.1 is an antibody against Clathrin Heavy Chain for use in Western Blotting, Flow Cytometry, Immunocytochemistry.

Flow Cytometry Analysis: 1.5 µg of this antibody from a representative lot detected Clathrin Heavy Chain in 1X10E6 Jurkat cells.
Immunocytochemistry Analysis: 25.0 µg/mL from a representative lot detected Clathrin Heavy Chain in HeLa, A431, HUVEC, and NIH/3T3 cells.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Clathrin Heavy Chain in 10 µg of PC12 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable