MABC1800

Anti-EZHIP Antibody, clone 1G11

• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 1G11, monoclonal
• Mol-Gew.: calculated mol wt 51.89 kDa; observed mol wt ~60 kDa
• Aufgereinigt durch: using protein G
• Speziesreaktivität: human
• Verpackung: antibody small pack of 100 μg
• Methode(n): immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG2aκ
• Epitopsequenz: N-terminal half
• Protein-ID-Hinterlegungsnummer: NP_981952
• UniProt-Hinterlegungsnummer: Q86X51
• Versandbedingung: 2-8°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... EZHIP(340602)

Beschreibung

Allgemeine Beschreibung

EZH inhibitory protein (UniProt: Q86X51; also known as EZHIP) is encoded by the EZHIP (also known as CXorf67) gene (Gene ID: 340602) in human. EZHIP is expressed predominantly in the gonads. In testis, it is detected in male germ cells inside the seminiferous tubules, especially in spermatogonia and round spermatids. In the ovary, it is expressed in primordial follicles and oocytes but not the external follicle cells. It is shown to inhibit PRC2/EED-EZH1 and PRC2/EED-EZH2 complex function by inhibiting EZH1/EZH2 methyltransferase activity, thereby causing down-regulation of histone H3 trimethylation on lysine 27 (H3K27me3). It inhibits H3K27me3 deposition during spermatogenesis and oogenesis. Its inhibitory effect on methyltransferase activity is attributed to its ability to limit the stimulatory effect of cofactors such as AEBP2 and JARID2. Amino acid sequence 403-423 is reported to be essential and sufficient for inhibition of PRC2/EED-EZH1 and PRC2/EED-EZH2 complex activity and the sequence 401-409 is sufficient for its interaction with EZH2. (Ref.: Ragazzini, R., et al. (2019). Nat. Commun. 10(1); 3858; Jain, SU., et al. (2019). Nat. Commun. 10(1); Article 2146).

Spezifität

Clone 1G11 is a mouse monoclonal antibody that detects EZH inhibitory protein. It targets an epitope within the N-terminal half.

Immunogen

His-tagged recombinant fragment corresponding to 145 amino acids from the N-terminal half of human EZH inhibitory protein (EZHIP).

Anwendung

Quality Control Testing

Evaluated by Western Blotting in Daoy cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected EZHIP in Daoy cell lysates.

Tested Applications

Immunofluorescence Analysis: A 1:1500 dilution from a representative lot detected EZHIP in Daoy cells.

Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated EZHIP in Daoy cell lysate.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-EZHIP, clone 1G11, Cat. No. MABC1800, is a mouse monoclonal antibody that detects EZH inhibitory protein (EZHIP) and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Physikalische Form

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1