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MAB2610

Sigma-Aldrich

Anti-Zyxin Antibody, clone 14E11.1

clone 14E11.1, from mouse

Synonym(e):

Zyxin2, Zyxin, Zyxin-2

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

14E11.1, monoclonal

Speziesreaktivität

human, mouse, rat

Methode(n)

immunocytochemistry: suitable
western blot: suitable

Isotyp

IgMκ

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

mouse ... Zyx(22793)

Allgemeine Beschreibung

Zyxin, is a cytosolic, focal adhesion protein belonging to the Ajuba family and containing 3 LIM zinc-binding domains. It associates with the actin cytoskeleton near the adhesion plaques and, during mitosis, with microtubules. Zyxin localizes to stress fibers, cell-cell junctions and colocalizes with vinculin, paxillin and VASP at focal adhesions but not at the tips of lamellipodia. Zyxin is involved in the regulation of actin dynamics and is a likely component of the signal transduction pathway mediating adhesion-stimulated changes in gene expression. Zyxin displays an actin nucleating activity that is dependent on its amino terminal proline-rich motifs and independent of Arp2/3 complex. Zyxin appears to function as an adaptor or docking protein to organize protein complexes important for regulated actin cytoskeleton dynamics.

Immunogen

KLH-conjugated linear peptide corresponding to human Zyxin.

Anwendung

Research Category
Zellstruktur
Research Sub Category
Cytoskelettale Signalübertragung
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Zyxin in NIH/3T3, A431, and HeLa cells.
This Anti-Zyxin Antibody, clone 14E11.1 is validated for use in WB, IC for the detection of Zyxin.

Qualität

Evaluated by Western Blot in HUVEC cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Zyxin in 10 µg of HUVEC cell lysate.

Zielbeschreibung

~84 kDa observed. A non-specific band may be observed at ~36 kDa
The calculated molecular weight is 61 kDa Zyxin is a phosphoprotein with an apparent molecular weight of 82–84 kDa (van der Gaag, E., et al. (2002). Journal of Investigative Dermatology. 118:246–254.). As a substrate of caspases, Zyxin can be cleaved as 30, 37, 48 and 70 kDa (C-B Chan, C-B., et al. (2007). Cell Death and Differentiation. 14:1688–1699.)

Physikalische Form

Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
HUVEC cell lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Zhen Xu et al.
International journal of molecular sciences, 25(4) (2024-02-24)
Focal adhesions (FAs) play a crucial role in cell spreading and adhesion, and their autophagic degradation is an emerging area of interest. This study investigates the role of Thrombospondin Type 1 Domain-Containing Protein 1 (THSD1) in regulating autophagy and FA

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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