FCMAB230P

Milli-Mark^® Mouse IgG1-k, clone MOPC-21, PE conjugate

This Milli-Mark Anti-mouse IgG1-PE Antibody, clone MOPC-21 is validated for use in FC for the detection of mouse IgG1.

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: PE
• Clone: MOPC-21, monoclonal
• Application: FACS
• Citations: 2

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: PE
• antibody form: purified immunoglobulin
• clone: MOPC-21, monoclonal
• species reactivity: human
• manufacturer/tradename: Milli-Mark^®
• technique(s): flow cytometry: suitable
• isotype: IgG1κ
• shipped in: wet ice
• target post-translational modification: unmodified

Beschreibung

General description

The MOPC-21 tumor line that produces mouse IgG1,k, is a mineral oil induced plasmacytoma. The product is originated and carried intraperitoneally in BALB/c mice. Identity and purity of the immunoglobulin is established by immunoelectrophoresis (IEP) and Ouchterlony Double Diffusion (ODD), prior to conjugation. Electrophoresis of the purified immunoglobulin, followed by diffusion versus anti-mouse whole serum, antimouse IgG1 and anti-mouse kappa chain, results in single arcs of precipitation. By ODD the purified immunoglobulin preparation is non-reactive with antisera to mouse lambda light chain, mouse IgA, IgM, IgG2a, IgG2b, and IgG3. The specificity of staining by monoclonal antibodies to human CD antigens should be verified by establishing the amount of non-specific binding to the target cell population. It is recommended that a non-reactive immunoglobulin of the same isotype, concentration and F/P molar ratio be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Isotype Control Antibodies

Biochem/physiol Actions

This mouse IgG1 isotype control antibody is not reactive with any known antigen.

Physical form

Immunoelectrophoresis

Purified mouse monoclonal IgG1κ conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Preparation Note

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Evaluated for use as flow cytometry isotype control using Jurkat Cells.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• WGK: WGK 2