CP11L

Anti-Integrin α₃ Mouse mAb (P1B5)

lyophilized, clone P1B5, Calbiochem^®

• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: P1B5, monoclonal
• Form: lyophilized
• Enthält nicht: preservative
• Speziesreaktivität: human
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Isotyp: IgG1
• Versandbedingung: ambient
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ITGA3(3675)

Beschreibung

Allgemeine Beschreibung

Purified mouse monoclonal antibody generated by immunizing RBF/Dn mice with the specified immunogen and fusing splenocytes with NS-1/FOX-NY mouse myeloma cells (see application references). Recognizes the α3 integrin subunit.

Recognizes the α₃-subunit of the α₃/β₁ integrin complex protein in human mononuclear cells.

This Anti-Integrin α₃ Mouse mAb (P1B5) is validated for use in Cell Inhibition Assays, FC, IF, IP for the detection of Integrin α₃.

Immunogen

Human

human mononuclear cells

Anwendung

Cell Inhibition Assays (1-5 µg/ml, see application references)

Flow Cytometry (1-5 µg/ml)

Immunoblotting (not recommended)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-2 µg/sample)

Paraffin Sections (not recommended)

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

Lyophilized from a volatile buffer, 100 µg BSA.

Rekonstituierung

We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage (with azide) or aliquot and freeze (-20°C) for short-term storage.

Hinweis zur Analyse

Positive Control
A2058 cells

Sonstige Hinweise

Albelda, S.M. and Buck, C.A. 1990. FASEB J.4, 2868.
Cheresh, D.A. et al. 1990. Cell57, 59.
Krissansen, G.W. et al. 1990. J. Biol. Chem.265, 823.
Vogel, B, et al. 1990. J. Biol. Chem.265, 5934.
Wayner, E.A. et al.1988 J. Cell Biol.107, 1881.
Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol.3, 179.
Hynes, R.O. 1987 Cell48, 549.
Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol.105, 1873.

Will partially inhibit cell-cell adhesion of E1L-8 cells. Antibody should be titrated for optimal results in individual systems.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable