CC161

ECMatrix-511 Silk E8 Laminin Substrate

Xeno-free laminin-511 coating for feeder-free pluripotent stem cell cultures, 1050 μg (Silkworm derived)

• UNSPSC Code: 12352200

Eigenschaften

• Produktname: ECMatrix-511 Silk E8 Laminin Substrate, Xeno-free laminin-511 coating for feeder-free pluripotent stem cell cultures, 1050 μg (Silkworm derived)

Beschreibung

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

• Animal-free, xeno-free format: Consistent from lot-to-lot with no prescreening required
• No plate precoating required: Save time by simply adding to media while passaging cells
• Supports single cell passaging w/out ROCKi: Great for CRISPR editing or clonal isolation
• Higher adhesion and growth rates: Get to your experiments faster
• Easy to handle: No chilling of cell culture consumables required

General description

Human pluripotent stem cells (ES and iPS cells) express α6β1 as the major integrin species and therefore can be maintained stably and expanded efficiently in feeder-free conditions on culture vessels coated with it′s binding partner laminin-511. However, laminin-511 is not suitable for large-scale production because of its large molecular weight and heterotrimeric nature. Professor Kiyotoshi Sekiguchi′s group (Matrixome, Inc.) have solved this problem by producing a recombinant E8 fragment of laminin-511 at large-scale while retaining the full integrin binding activity. The ECMatrix-511 E8 Laminin Substrate can be used to culture pluripotent stem cells in feeder-free conditions with numerous added benefits over traditional methods.

Preparation Note

ECMatrix-511 E8 Laminin Substrates should be stored at 2-8°C. Avoid multiple freeze-thaw cycles and protect from light.