AP501A

Goat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, Alkaline Phosphatase, Species Adsorbed

Chemicon^®, from goat

• UNSPSC Code: 12352203
• NACRES: NA.46
• eCl@ss: 32160702
• Conjugate: alkaline phosphatase conjugate
• Clone: polyclonal
• Application: ELISA
• Citations: 1

Eigenschaften

• biological source: goat
• Quality Segment: 100
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity purified immunoglobulin
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: mouse
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable
• shipped in: dry ice
• target post-translational modification: unmodified

Beschreibung

General description

IgG is the most abundant immunoglobulin compose of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

Application

ELISA: 1:2,000-1:4,000

Optimal working dilutions must be determined by the end user.

Goat anti-Mouse IgM+IgG+IgA (H+L) Antibody, Alkaline Phosphatase, Species Adsorbed detects level of Mouse IgM+IgG+IgA & has been published & validated for use in ELISA.

Biochem/physiol Actions

Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.

Physical form

Purified by affinity chromatography on pooled mouse IgM+IgG+IgA covalently linked to agarose. Liquid in 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative.

Preparation Note

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. Store long term at -20°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1