662098

UbcH5c Conjugating Enzyme E2, Human, Recombinant, E. coli

• Synonym(e): UBD2D3, Ubiquitin-conjugating enzyme E2 D3
• UNSPSC Code: 12352203
• Form: solution
• Assay: ≥95% (SDS-PAGE)

Eigenschaften

• assay: ≥95% (SDS-PAGE)
• Quality Segment: 100
• form: solution
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Beschreibung

General description

Full-length, recombinant, human UbcH5c expressed in E. coli. UbcH5c, an E2 ubiquitin ligase, is highly homologous to the yeast UbC4/5 family of E2 enzymes. UbcH5c, together with UbcH5a and UbcH5b, are the most active class of E2 enzymes in cell extracts and are associated with regulation of transcription factors such as p53, NF-κB3, and c-Fos. The active enzyme is useful in the study of ubiquitin regulation and inhibitor screening.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM HEPES, 50 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.0.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Brzovic, P.S., et al. 2006. Cell Cycle5, 2867.
Gehrke, S.G., et al. 2003 Blood.101, 3288.
Nuber, U., And Scheffner, M., 1999. J. Biol. Chem.274, 7576.
Schwarz, S.E., et al. 1998. J. Biol. Chem.273, 12148.
Jensen, J., et al. 1995. J. Biol. Chem.270, 30408.
Scheffner, M., et al. 1994. Proc. Natl. Acad. Sci.91, 8797.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 1