Product 648314, a discontinued item, is a 1.0 M Tris buffer solution, pH 8.0, molecular biology grade, supplied as a sterile liquid and based on Tris/Tris base. Product T6664 is Tris Buffered Saline (TBS) powder; when one pouch is dissolved in 1 L water, the result is 0.05 M Tris buffered saline containing NaCl 0.138 M and KCl 0.0027 M. Therefore, this product contains saline components and is more diluted in Tris after reconstitution, whereas this product is a 1.0 M Tris buffer solution, so they are not compositionally equivalent.
648314
Tris-Puffer, 1,0 M, pH 8,0, molekularbiologische Qualität
1.0 M liquid, pH 7.0-9.0, Molecular Biology Grade
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(e):
Tris(hydroxymethyl)aminomethan, 2-Amino-2-(hydroxymethyl)-1,3-propandiol, THAM, Tris Base, Trometamol
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Über diesen Artikel
Lineare Formel:
NH2C(CH2OH)3
CAS-Nummer:
Molekulargewicht:
121.14
UNSPSC Code:
12161705
NACRES:
NA.25
MDL number:
Beilstein/REAXYS Number:
741883
Produktname
Tris-Puffer, 1,0 M, pH 8,0, molekularbiologische Qualität, Sterile solution. Suitable for DNA and RNA applications. Tris buffer is useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
grade
Molecular Biology
Quality Segment
description
RTECS - TY2900000
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
clear colorless
useful pH range
7-9
pKa (25 °C)
8.1
bp
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.)
foreign activity
DNases, RNases, none detected
shipped in
ambient
storage temp.
10-30°C
SMILES string
NC(CO)(CO)CO
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key
LENZDBCJOHFCAS-UHFFFAOYSA-N
General description
Sterile Lösung. Geeignet für DNA- und RNA-Anwendungen. Für die Anwendung im pH-Bereich 7,0–9,0. Der pKa-Wert bei 25 °C beträgt 8,1.
Application
- Physicochemical cell disruption of Bacillus sp. for recovery of polyhydroxyalkanoates: future bioplastic for sustainability.: This research used Tris buffer, pH 8.0, for cell lysis and extraction processes, contributing to sustainable bioplastic production through efficient recovery of polyhydroxyalkanoates (Bhat et al., 2024).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxizität: Reizstoff (B)
Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 1
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Dear Sir, Are the compositions of this product the same with T6664 Tris buffer(powder form)?
1 answer-
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How is this product sterilized?
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This product is sterilized by filtering the solution through a 0.2 micron filter.
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Is Tris Buffer, 1.0 M, pH 8.0 the same as 1XTE?
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A 1 M Tris buffer solution is not equivalent to a 1 X Tris-EDTA (TE) buffer preparation. The composition of 1X TE buffer is 10mM TRIS-HCl and 1mM EDTA. Please be aware there are Tris-EDTA buffer solutions offered in the product portfolio. These options that are pH 8.0 are products 93283 and 8890. Please see the corresponding product information page for both of these items at the links below:
https://www.sigmaaldrich.com/product/sial/93283
https://www.sigmaaldrich.com/product/mm/8890opHelpful?
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