524739

Phosphodiesterase 10A2, His•Tag^®, Human, Recombinant, S. frugiperda

• Synonym(e): PDE10A2
• EG-Nummer: 3.1.4.17 (BRENDA, IUBMB)
• NACRES: NA.54
• UNSPSC Code: 12352202
• Specific activity: ≥0.25 U/μL
• Recombinant: expressed in baculovirus infected Sf21 cells

Eigenschaften

• recombinant: expressed in baculovirus infected Sf21 cells
• Quality Segment: 100
• form: liquid
• specific activity: ≥0.25 U/μL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Beschreibung

General description

Full-length, recombinant, human, phosphodiesterdase 10A2 (accession no. 10A2) fused at the N-terminus to a His•Tag^7reg; sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified preparation of catalytically active enzyme.

Physical form

In RIPA buffer (150 mM NaCl, 10 mM Tris-HCl, 1% NP-40), pH 8.3.

Other Notes

Kotera, J., et. al. 2004. J. Biol. Chem.279, 4366.
Fujishige K., et. al. 1999. Eur. J. Biochem.266, 1118.
Kotera, J., et. al. 1999. Biochem. Biophys. Res. Commun.261, 551.

One unit is defined as the amount of enzyme required to convert 1 µmol cAMP to AMP per min at 30°C, pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Signalwort: Danger
• Gefahrencodes: EUH430
• Sicherheitshinweise: P201 - P202 - P273 - P391 - P405 - P501
• Hazard Classifications: ED ENV 1