475879

Monastrol

A potent, cell-permeable, non-tubulin-interacting mitosis inhibitor.

• Synonym(e): Monastrol, 4-(3-Hydroxyphenyl)-6-methyl-2-thioxo-1,2,3,4-tetrahydro-4H-pyrimidin-5-carboxylic Acid Ethyl Ester, Eg5 Inhibitor I
• Empirische Formel (Hill-System): C₁₄H₁₆N₂O₃S
• CAS-Nummer: 254753-54-3
• Molekulargewicht: 292.35
• UNSPSC Code: 12352200
• NACRES: NA.32
• MDL number: MFCD00813077
• Assay: ≥95% (HPLC)
• Form: solid
• Storage condition: OK to freeze, protect from light

Eigenschaften

• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: white
• solubility: DMSO: 100 mg/mL
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: S=C1N[C@H](C(=C(N1)C)C(=O)OCC)c2cc(ccc2)O
• InChI: 1S/C14H16N2O3S/c1-3-19-13(18)11-8(2)15-14(20)16-12(11)9-5-4-6-10(17)7-9/h4-7,12,17H,3H2,1-2H3,(H2,15,16,20)/t12-/m0/s1
• InChI key: LOBCDGHHHHGHFA-LBPRGKRZSA-N

Beschreibung

General description

A potent, cell-permeable, non-tubulin-interacting mitosis inhibitor. Arrests mammalian cells in mitosis with monopolar spindles. In vitro, it specifically inhibits the motility of the mitotic kinesin Eg5, a motor protein involved in the assembly and maintenance of the mitotic spindle (IC₅₀ = 14 µM).

A potent, cell-permeable, nontubulin-interacting mitosis inhibitor. Arrests mammalian cells in mitosis with monopolar spindles. In vitro, it specifically inhibits the motility of the mitotic kinesin Eg5, a motor protein involved in the assembly and maintenance of the mitotic spindle (IC₅₀ = 14 µM).

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Mitotic kinesin Eg5

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 14 µM against mitotic kinesin Eg5

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Kapoor, T.M., et al. 2000. J. Cell Biol.150, 975.
Mayer, T.U., et al. 1999. Science286, 971.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable