371723

Anti-G_iα-1 and G_iα-2-Subunits, C-Terminal (345-354 and 346-355) Rabbit pAb

liquid, Calbiochem^®

• UNSPSC Code: 12352203
• Clone: polyclonal
• Species reactivity: -
• Application: —
• Citations: 11

Eigenschaften

• biological source: rabbit
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• does not contain: preservative
• species reactivity (predicted by homology): mammals
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• isotype: IgG
• shipped in: wet ice
• storage temp.: −70°C
• target post-translational modification: unmodified
• Gene Information: human ... GNAI1(2770)

Beschreibung

General description

Anti-Giα-1 and Giα-2-Subunits, C-Terminal (345-354 and 346-355), rabbit polyclonal, recognizes Giα-1 and Giα-2 subunits. It is validated for use in Western blotting.

Protein A purified rabbit polyclonal antibody. Recognizes G_iα-1 and G_iα-2 subunit proteins.

Recognizes G_iα-1 and G_iα-2 subunits. Does not cross-react with G₃α-3 or G_oα subunits.

Immunogen

a synthetic peptide [(C)KNNLKDCGLF] corresponding to amino acids at the C-terminus of mammalian G_iα-1 and G_iα-2, conjugated to KLH

Application

Immunoblotting (1:1000)

Physical form

In 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Does not cross-react with G_iα-3 and G_oα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for G_iα-1, G_iα-2, G_iα-3, and G_oα. Variables associated with assay conditions will dictate the proper working dilution.

Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.
Goldsmith, P.P., et al. 1987. J. Biol. Chem.262, 14683.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 1