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371207

Dkk-1, Mouse, Recombinant

Name: Dkk-1, Mouse, Recombinant
Brand: MM

Dkk-1, Mouse, Recombinant, consists of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.

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Über diesen Artikel

NACRES:

NA.41

UNSPSC Code:

12352203

Form:

liquid

Assay:

≥95% (SDS-PAGE)

Recombinant:

expressed in CHO cells

Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Technischer Dienst

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Unterstützung erhalten

Eigenschaften

recombinant

expressed in CHO cells

Quality Segment

100

assay

≥95% (SDS-PAGE)

form

liquid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

impurities

<1.0 EU/μg protein Endotoxin

shipped in

ambient

storage temp.

−20°C

Beschreibung

General description

Recombinant, mouse Dickkopf related protein 1 (Dkk-1) consisting of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. ND₅₀ = 0.02-0.1 µg/ml.

Biochem/physiol Actions

ND₅₀ = 0.02-0.1 µg/ml, in the presence of 10 ng/ml of rmWnt-3a, as measured by its ability to inhibit Wnt-3a-inducedalkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.

Physical form

Lyophilized from 0.2 µm sterile-filtered PBS, BSA.

Preparation Note

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml in PBS containing at least 0.1% human or bovine serum albumin.

Other Notes

Pinzone, J.J., et al. 2009. Blood113, 517.
Li, J., et al. 2006. Bone36, 754.
Morvan, F., et al. 2006. J. Bone Miner. Res.21, 934.
Niehrs, C., 2006. Oncogene25, 7469.
Verani, R., et al. 2006. J. Neurochem.101, 242.
Cappuccio, I., et al. 2005. J. Neurosci.25, 2647.
Kemp, C., et al. 2005. Dev. Dyn.233, 1064.
Adamska, M., et al. 2004. Dev. Biol.272, 134.
Bullock, C.M., et al. 2004. Mol. Pharmacol.65, 582.
Mao, B., et al. 2002. Nature417, 664.
Mao, B., et al. 2001. Nature411, 321.
Glinka, A., et al. 1998. Nature391, 357.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Dieser Artikel	371208	SRP3258	567693
Sigma-Aldrich 371207 Dkk-1, Mouse, Recombinant	Sigma-Aldrich 371208 Dkk-1, Rat, Recombinant	Sigma-Aldrich SRP3258 DKK-1 human	Sigma-Aldrich 567693 SOST, Mouse, Recombinant
description Dkk-1, Mouse, Recombinant, consists of amino acids 30-272 and expressed in Chinese Hamster Ovary cells. Inhibits Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells.	description Dkk-1, Rat, Recombinant, consists of amino acids 23-270, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.	description recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture	description -
recombinant expressed in CHO cells	recombinant expressed in mouse NSO cells	recombinant expressed in HEK 293 cells	recombinant -
assay ≥95% (SDS-PAGE)	assay ≥95% (SDS-PAGE)	assay ≥97% (HPLC), ≥97% (SDS-PAGE)	assay ≥90% (SDS-PAGE)
form liquid	form lyophilized	form lyophilized	form solid
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
impurities <1.0 EU/μg protein Endotoxin	impurities ≤1.0 EU/μg protein Endotoxin	impurities <0.1 EU/μg endotoxin, tested	impurities <1.0 EU/μg protein Endotoxin
shipped in ambient	shipped in ambient	shipped in wet ice	shipped in ambient

Lagerklasse

11 - Combustible Solids

wgk

WGK 3

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