345763

GDF-9, Mouse, Recombinant

GDF-9, Mouse, Recombinant, consists of amino acids 307-477 and is expressed in Chinese Hamster Ovary cells. An oocyte secreted paracrine factor that is necessary for mammalian folliculogenesis.

• UNSPSC-Code: 12352203

Eigenschaften

• Rekombinant: expressed in CHO cells
• Qualitätsniveau: 100
• Assay: ≥95% (SDS-PAGE)
• Form: solid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Verunreinigungen: <1.0 EU/μg protein Endotoxin
• Versandbedingung: ambient
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Recombinant, mouse growth differentiation factor 9 (GDF-9) consisting of amino acids 307-477 and expressed in Chinese Hamster Ovary cells. GDF-9 is an oocyte secreted paracrine factor, necessary for mammalian ovarian folliculogenesis. Mature mouse GDF-9 is 90% identical to the amino acid sequence of mature human GDF-9. GDF-9 as a paracrine factor is useful in granulosa cell proliferation and differentiation regulation.

Biochem./physiol. Wirkung

Effective concentration = 100 ng/ml as measured by induction of Smad2 phosphorylation in P19 mouse embryonal carcinoma cells.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

Lyophilized from 0.2 µm sterile-filtered HCl, BSA.

Rekonstituierung

Reconstitute to 100 µg/ml in 4 mM HCl containing 0.1% bovine serum albumin.

Sonstige Hinweise

Mottershead, D.G., et al. 2008. Mol. Cell. Endocrinol.283, 58.
Gilchrist, R.B., et al. 2006. J. Cell. Sci.119, 3811.
Mazerbourg, S., et al. 2004. Mol. Endocrinol.18, 653.
Kaivo-Oja, N., et al. 2003. J. Clin. Endocrinol. Metab.88, 755.
Roh, J.S., et al. 2003. Endocrinology144, 172.
Vitt, U.A., et al. 2002. Biol. Reprod.67, 473.
Dong, J., et al. 1996. Nature383, 531.
McGrath, S.A., et al. 1995. Mol. Endocrinol.9, 131.
McPherron, A.C. and S.J. Lee. 1992. J. Biol. Chem.268, 3444.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3