230914

Chymotrypsin Substrate II

≥98% (HPLC), fluorogenic chymotrypsin substrate, lyophilized solid

• Synonym(e): Chymotrypsin-Substrat II, fluorogen
• Empirische Formel (Hill-System): C₃₄H₃₉N₅O₉
• CAS-Nummer: 88467-45-2
• Molekulargewicht: 661.70
• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD00080246
• Assay: ≥98% (HPLC)
• Form: lyophilized solid
• Storage condition: OK to freeze, desiccated, protect from light

Eigenschaften

• Produktname: Chymotrypsin-Substrat II, fluorogen, Sensitive fluorogenic substrate for the quantitative determination of chymotrypsin activity.
• Quality Segment: 100
• assay: ≥98% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated, protect from light
• color: white
• solubility: DMSO: soluble, acetonitrile: water (1:1): soluble
• fluorescence: λ_ex ~380 nm, λ_em ~460 nm
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: N1([C@@H](CCC1)C(=O)NC(=O)[C@@H](Nc3cc4c(cc3)C(=CC(=O)O4)C)Cc2ccccc2)C(=O)[C@@H](NC(=O)[C@@H](NC(=O)CCC(=O)O)C)C
• InChI: 1S/C34H39N5O9/c1-19-16-30(43)48-27-18-23(11-12-24(19)27)37-25(17-22-8-5-4-6-9-22)32(45)38-33(46)26-10-7-15-39(26)34(47)21(3)36-31(44)20(2)35-28(40)13-14-29(41)42/h4-6,8-9,11-12,16,18,20-21,25-26,37H,7,10,13-15,17H2,1-3H3,(H,35,40)(H,36,44)(H,41,42)(H,38,45,46)/t20-,21-,25-,26-/m0/s1
• InChI key: IGKPQFYMWKQDQS-KCXKOMAXSA-N

Beschreibung

General description

Sensitives fluorogenes Substrat für die quantitative Bestimmung der Aktivität von Chymotrypsin (Anregungsmaximum: ~380 nm; Emissionsmaximum: ~460 nm).

Biochem/physiol Actions

Zellpermeabel: nein

Primärziel
Chymotrypsin

Produkt konkurriert nicht mit ATP.

Reversibel: nein

Preparation Note

Nach der Rekonstitution in Aliquote aufteilen und einfrieren (-20 °C). Wiederholte Einfrier-Auftau-Zyklen der Lösungen vermeiden. Stammlösungen sind bei -20 °C bis zu 3 Monate haltbar.

Other Notes

Irvine, G.B., et al. 1990. Anal. Biochem.185, 304.
Graf, L., et al. 1987. Biochemistry26, 2616.
Oshima, G. 1984. J. Biochem.95, 1131.
Oshima, G. 1983. J. Biochem.94, 1615.

Suc-Ala-Ala-Pro-Phe-AMC

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxizität: Standard-Handhabung (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable