23-048-M

Parkin Protein, active, 10 µg

Active, full length, human recombinant human Parkin. N-terminal 6His-tagged. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.

• Synonym(e): Parkinson juvenile disease protein 2, PARK2, PRKN
• UNSPSC Code: 12352202
• NACRES: NA.41
• eCl@ss: 32160405
• Biological source: human
• Recombinant: expressed in Sf21 cells

Eigenschaften

• biological source: human
• Quality Segment: 100, 300
• recombinant: expressed in Sf21 cells
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable
• NCBI accession no.: NM_004562

Beschreibung

General description

Parkin is an E3-ubiquitin ligase belonging to the RBR (RING – In Between RING – RING) family. Mutations in Parkin that result in a loss-of-function are the most frequent cause of familial Parkinson’s disease and are responsible for a large percentage of autosomal recessive juvenile parkinsonism (AR-JP). In addition to its auto-ubiquitination, Parkin can also ubiquitinate various substrates, including the CDK2-interacting protein cyclin E1, the a-synuclein-interacting protein synphilin-1, the synaptic vesicle-associated CDC-rel1 and the p38 subunit of aminoacyl-tRNA synthetase/JTV-1. Ubiquitination of substrates occurs in conjunction with the E2 enzymes UbcH7, UbcH8 or UbcH13/Uev1.

Application

Ubiquitination Cascade Comoponent: E3

Physical form

Purified using immobilized metal affinity chromatography.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• Gefahrencodes: H317
• Sicherheitshinweise: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Lagerklasse: 12 - Non Combustible Liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable