219467

Cathepsin V, His•Tag^®, Human, Recombinant, NSO Cells

recombinant human, expressed in NSO cells

• Synonym(e): Cathepsin L2, Cathepsin U
• EC-Nummer: 3.4.22.43 (BRENDA, IUBMB)
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Rekombinant: expressed in NSO cells
• Qualitätsniveau: 100
• Assay: ≥95% (SDS-PAGE)
• Form: frozen liquid
• Spezifische Aktivität: ≥300 units/μg
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Verunreinigungen: ≤1.0 EU/μg Endotoxins (EU/μg Cathepsin V)
• Versandbedingung: wet ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Recombinant, human procathepsin V (amino acids 18-334) fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His•Tag sequence and expressed in NSO cells. Cat V is predominantly expressed in normal thymus, testis, and corneal epithelium. It is involved in the invariant chain degradation in thymic epithelial cells and its overexpression has been detected in myasthenia gravis. Abnormally wide spread expressions in mammary gland and colon have also been reported in breast and colonrectal carcinomas. Although it shows high amino acid identity with cathepsin L (Cat. No. 219382), these two cathepsins have very different tissue distribution and substrate specificity. As a result of glycosylation, this recombinant enzyme migrates ~40 kDa on SDS-PAGE under either reducing or non-reducing conditions. Requires activation prior to use.

Recombinant, human procathepsin V (amino acids 18-334) fused at the N-terminus to a CD33 signal sequence (amino acids 1-16) and at the C-terminus to a His•Tag sequence and expressed in NSO cells. Cat V is predominantly expressed in normal thymus, testis, and corneal epithelium. It is involved in the invariant chain degradation in thymic epithelial cells and its overexpression has been detected in myasthenia gravis. Abnormally wide spread expressions in mammary gland and colon have also been reported in breast and colonrectal carcinomas. Although it shows high amino acid identity with cathepsin L (Cat. No. 219382), these two cathepsins have very different tissue distribution and substrate specificity. As a result of glycosylation, this recombinant enzyme migrates ~40 kDa on SDS-PAGE under either reducing or non-reducing conditions. Requires activation prior to use.

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Einheitendefinition

One unit is defined as the amount of enzyme that will cleave 1 pmol the fluorescent substrate Z-LR-AMC per min at 25°C, pH 5.5.

Physikalische Form

In 800 mM NaCl, 25 mM sodium acetate, 0.8 M NaCl, pH 5.0.

Rekonstituierung

To activate rhCathepsin V, pre-incubate it at 20 µg/ml in 25 mM NaOAc, 100 mM NaCl, 5 mM DTT, pH 5.5 at room temperature. Following initial thaw aliquot and freeze (-70°C).

Sonstige Hinweise

Tolosa, E., et al. 2003. J. Clin. Invest.112, 517.
Turk, V., et al. 2001. EMBO J.20, 4629.
Somoza, J.R., et al. 2000. Biochemistry39, 12543.
Bromme, D., et al. 1999. Biochemistry38, 2377.
Adachi, W., et al. 1998. Invest. Ophthalmol. Vis. Sci.39, 1789.
Santamaria, I., et al. 1998. Cancer Res.58, 1624.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable