1.50635

LiChrospher^® RP-8 (5 µm) HPLC Columns

L x I.D. 150 mm x 4.6 mm, LiChroCART^™ HPLC cartridge, manuCART^™ holder required for use

• UNSPSC Code: 41115709
• NACRES: SB.52
• Particle size: 5 μm
• Matrix active group: C8 (RP8), C8 bonding phase
• Pore size: 100 Å pore size
• Matrix: fully porous particle

Eigenschaften

• Produktname: LiChrospher^® RP-8 (5µm) LiChroCART^™ 150-4.6, suitable for HPLC
• material: stainless steel column
• Quality Segment: 100
• agency: suitable for USP L7
• product line: LiChrospher^®
• feature: endcapped: no
• extent of labeling: 12.5% Carbon loading
• parameter: 250 bar pressure, 60 °C max. temp.
• technique(s): HPLC: suitable, mass spectrometry (MS): suitable (Low - Medium)
• column L × I.D.: 15 cm × 4.6 mm
• surface area: 350 m²/g
• matrix: fully porous particle
• matrix active group: C8 (RP8), C8 bonding phase
• particle size: 5 μm
• pore size: 100 Å pore size
• operating pH range: 2-7.5
• separation technique: reversed phase
• storage temp.: no temp limit

Beschreibung

General description

HPLC cartidge

LiChrospher^® RP-8 is a reliable and versatile traditionally produced spherical silica gel carrier with C-8 reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds. This sorbent is packed into a LiChroCART 150-4.6 HPLC cartridge. The connection to the HPLC system is being accomplished by use of the manu-CART NT cartrige holder (1.51486).

Application

• Stability studies of crude plant material of Bacopa monnieri and quantitative determination of bacopaside I and bacoside A by HPLC.: This study focuses on the stability of crude plant material from Bacopa monnieri, specifically analyzing the stability of its active constituents, bacopaside I and bacoside A. High-performance liquid chromatography (HPLC) using Purospher^® RP-18 (5 µm) columns was employed for the quantitative determination. The findings highlighted the efficacy of Purospher^® RP-18 columns in separating and quantifying these compounds with high precision, making it a valuable tool in analytical chemistry for phytochemical studies (Srivastava et al., 2012).

Analysis Note

Pressure: ≤ 50 bar
Theoretical Plates (N/m) (Toluene): ≥ 50000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 0.8 - 2.0

Legal Information

LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany

LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany