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Merck

1.50471

Supelco

Purospher® STAR RP-18 endcapped (3 µm) HPLC Columns

L × I.D. 250 mm x 4.6 mm Hibar® RT HPLC Column

Synonym(e):

Purospher® STAR RP-18 endcapped (3µm) Hibar® RT 250-4.6

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About This Item

UNSPSC-Code:
41115709
NACRES:
SB.52

product name

Purospher® STAR RP-18 mit Endcapping (3 µm), L × I.D. 250 mm x 4.6 mm Hibar® RT HPLC column

Materialien

stainless steel column

Qualitätsniveau

Agentur

suitable for USP L1

Produktlinie

Purospher®

Leistungsmerkmale

endcapped

Verpackung

1 ea of

Kennzeichnungsgrad

17% Carbon loading

Parameter

400 bar pressure
65 °C max. temp.

Methode(n)

HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)

Säule L × ID

25 cm × 4.6 mm

Oberflächenbereich

330 m2/g

Matrix

fully porous particle

Aktive Matrixgruppe

C18 bonding phase

Partikelgröße

3 μm

Porengröße

120 Å pore size

operating pH range

1.5-10.5

Trenntechnik

reversed phase
supercritical fluid (SFC)

Lagertemp.

no temp limit

Verwandte Kategorien

Allgemeine Beschreibung

Purospher® STAR RP-18 endcapped, 3 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Thanks to these features, Purospher® STAR RP-18 endcapped, 3 µm HPLC columns allow maximum flexibility to use the best chromatographic conditions for any separation including reversed phase.Purospher® STAR RP-18 endcapped, 3 µm Hibar® RT 250-4.6 is a ready to use stainless steel column suitable for connection to any HPLC system.The column dimension of 250 mm length x 4.6 mm I.D. is the optimal choice for high resolution HPLC separation of complex samples.The recommended guard column is Purospher® STAR RP-18 endcapped (5 µm) LiChroCART® 4-4 part number 1.50250.0001. For direct connection to a Hibar® RT columns the Precolumn holder for 4-4 LiChroCART® cartridges, part number 1.51487.0001 can be used.Item 1.50471.1003 is a Validation kit containing 3 Purospher® STAR RP-18 endcapped, 3 µm Hibar® RT 250-4.6 HPLC columns from 3 different base material lots.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Anwendung

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Leistungsmerkmale und Vorteile

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

Hinweis zur Analyse

Druck: ≤ 280 bar
Theoretische Bodenzahl (N/m) (Anthracen): ≥ 120000
Symmetrie (Anthracen): 0.8 - 1.4
Kapazitätsfaktor (Anthracen): 4.0 - 6.0

Rechtliche Hinweise

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany
LICHROCART is a registered trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

HPLC method determines Drospirenone in progestin medication using Purospher® STAR RP-18e Hibar® HPLC column, meeting USP monograph criteria.

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