1.08218

Trifluoressigsäurelösung

(25% solution in water) for protein sequenation

• Synonym(e): Trifluoressigsäure -Lösung, TFA
• Lineare Formel: CF₃COOH
• CAS-Nummer: 76-05-1
• Molekulargewicht: 114.02
• NACRES: NB.21
• UNSPSC Code: 12352106
• Beilstein/REAXYS Number: 5446934
• MDL number: MFCD00004169
• Concentration: 24.5-25.5% (trifluoroacetic acid)
• Form: liquid

Eigenschaften

• Quality Segment: 200
• form: liquid
• composition: Water, 74.5-75.5% Karl Fischer
• concentration: 24.5-25.5% (trifluoroacetic acid)
• density: 1.12 g/cm³ at 20 °C
• storage temp.: 15-25°C
• SMILES string: OC(=O)C(F)(F)F
• InChI: 1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)
• InChI key: DTQVDTLACAAQTR-UHFFFAOYSA-N

Beschreibung

Application

Trifluoroacetic acid (TFA) can be used as a solvent in a reverse-phase HPLC system to separate peptides because it enhances peak resolution. It also has low absorption detection wavelengths and is relatively volatile and easily removed. For the majority of peptide separations, TFA has been used at concentrations ranging from 0.05 to 0.1%(6.5-13 mM).

Analysis Note

Gehalt (alkalimetrisch): 24.5 - 25.5 %
Identität (IR-Spektrum): entspricht
Dichte (d 20 °C/ 4 °C): 1.129 - 1.131
Fluoreszenz (bei 254 nm): ≤ 2 ppb
Fluoreszenz (bei 365 nm): ≤ 2 ppb
Wasser (nach Karl Fischer): 74.5 - 75.5 %

Sicherheitshinweise

• Piktogramme: GHS08,GHS05
• Signalwort: Danger
• Hazard Classifications: Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Corr. 1A
• Lagerklasse: 8B - Non-combustible corrosive hazardous materials
• supp_hazards: EUH071
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Gefahrencodes: H314,H361d,H412
• Sicherheitshinweise: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338