If sediment is noted at the bottom of the stock Leishman′s eosin methylene blue solution, Technical Service should be contacted and a no charge replacement requested. If the diluted Leishman′s eosin methylene blue solution forms a precipitate this is normal. Although the individual dyes used to prepare 105387 are water soluble, after the Leishman′s eosin methylene blue solution has been manufactured, the Leishman dye is no longer water soluble. Given time the dyes will start to precipitate out of solution. Once the staining performance starts to diminish, the diluted Leishman solution should be replaced with a fresh diluted solution.
1.05387
Leishman′s eosin methylene blue solution modified
for microscopy
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
500 mL | Warenkorb auf Verfügbarkeit prüfen | € 106,00 |
Über diesen Artikel
NACRES:
NA.05
UNSPSC Code:
41116121
Technischer Dienst
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Unterstützung erhaltenvapor pressure
128 hPa ( 20 °C)
Quality Segment
form
liquid
autoignition temp.
455 °C (methanol)
expl. lim.
5.5-44 % (v/v) methanol()
IVD
for in vitro diagnostic use
bp
65 °C/1013 hPa
transition temp
flash point 12 °C
density
0.79 g/cm3 at 20 °C
storage temp.
20-25°C
General description
The staining method according to Leishman is one of the standard techniques in hematological diagnostic procedures. Like Wright′s eosin methylene blue solution and May-Grünwald′s eosin methylene blue solution,this ready-to-use solution is used for staining of blood and bone marrow smears and clinical cytological specimens. The typical resulting color of cell nuclei is red to purple, which depends on the buffer tablets (Buffer tablets pH 7.2 or Buffer tablets pH 6.8). The staining of the nuclei is due of the molecular interaction of the Eosin Y dye and a complex of Azur B with DNA. Both dyes assemble to an Eosin Y - Azur B-DNA complex and the intensity of the resulting stain depends on the content of Azur B and the ratio of Azur B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances.
A 500 mL bottle of Leishman′s eosin methylene blue solution are sufficient for staining up to 1600 slides in Hellendahl cells. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
A 500 mL bottle of Leishman′s eosin methylene blue solution are sufficient for staining up to 1600 slides in Hellendahl cells. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Application
For microscopy. For staining of blood and bone marrow smears and clinical-cytological specimens
Analysis Note
Suitability for microscopy (Blood smear):passes test
Erythrocytes:reddish
Nuclei:violet
Eosinophilic granules:brick-red
Neutrophilic granules:light violet
Lymphocyte cytoplasm:blue
Erythrocytes:reddish
Nuclei:violet
Eosinophilic granules:brick-red
Neutrophilic granules:light violet
Lymphocyte cytoplasm:blue
Signalwort
Danger
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - Skin Sens. 1 - STOT SE 1
Zielorgane
Eyes,Central nervous system
Lagerklasse
3 - Flammable liquids
Was ist los?
WGK 2
Flammpunkt (°F)
53.6 °F
Flammpunkt (°C)
12 °C
Zulassungslistungen
Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.
67-56-1
CAS No.
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If a blue colour sedimentation occurs at the bottom , how to proceed or filter it. Membrane of filter and pore size is required.
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