06-1130

Anti-RNF168 Antibody

• Synonym(e): E3 ubiquitin-protein ligase RNF168, RING finger protein 168
• eCl@ss: 32160702
• UNSPSC Code: 12352203

Eigenschaften

• biological source: rabbit
• antibody form: affinity isolated antibody
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_689830
• UniProt accession no.: Q8IYW5

Beschreibung

General description

RNF168 is a ubiquitin ligand that binds to ubiquinated histone H2A and localizes to sites of DNA damage. RNF168-dependent chromatin modifications cause 53BP1 and BRCA1 to accumulated at the site. A mutation in RNF168 has been associated with RIDDLE syndrome, an immunodeficiency and radiosensitivity disorder. RNF168 is responsible for securing repair factors at sites of DNA damage. RNF168 has two MIU (motif interacting with ubiquitin) and one UMI (UIM- and MIU-related UBD) ubiquitin binding domains (UBDs) which are essential for proper localization of RNF168.

~79 kDa observed.

Immunogen

Histidine-tagged recombinant protein corresponding to the C-terminus of human RNF168.

Application

Anti-RNF168 Antibody is a Rabbit Polyclonal Antibody for detection of RNF168 also known as E3 ubiquitin-protein ligase RNF168 & has been validated in WB.

Immunoprecipitation Analysis: A representative lot of this antibody was used to immunoprecipitate RNF168 from whole cell lysates (Zhao, H., et al. (2014) Cell Cycle. 13(11):1–11).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Biochem/physiol Actions

This antibody recognizes RNF168 at the C-terminus.

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Jurkat cell lysate

Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 0.1 μg/mL of this antibody detected RNF168 on 10 μg of Jurkat cell lysate.

Other Notes

Concentration:Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Sicherheitshinweise

• Lagerklasse: 10-13 - German Storage Class 10 to 13