814449

TSKgel^® Boronate-5PW (10 µm) HPLC Columns

L x I.D. 5 cm × 5 mm, m-aminophenyl boronate phase

• Synonym(s): TSKgel^® Boronate-5PW HPLC Column
• UNSPSC Code: 41115711

Properties

• product name: TSKgel^® Boronate-5PW HPLC Column, phase m-aminophenyl boronate, L × I.D. 5 cm × 5 mm, 10 μm particle size, glass
• material: glass
• product line: TSKgel^®
• analyte chemical class(es): proteins
• manufacturer/tradename: Tosoh 14449
• parameter: ≤20% organic solvent; ≤3 M salt concentration; >0 °C temperature; 1.2 mL/min flow rate; 30 bar max. pressure
• technique(s): HPLC: suitable
• L × I.D.: 5 cm × 5 mm
• matrix: hydroxylated methacrylic polymer
• matrix active group: m-aminophenyl boronate phase
• particle size: 10 μm
• operating pH: 2-9
• capacity: 40 μmol/mL, resin adsorption capacity (sorbitol)
• separation technique: affinity
• storage temp.: 2-8°C

Description

General description

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.

Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation