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P8124

Sigma-Aldrich

Pyoverdines

from Pseudomonas fluorescens, >90% (HPLC)

Synonym(s):

Pyoverdine Detection, Pyoverdine Protein, Siderophore Protein

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

Pseudomonas fluorescens

Quality Level

Assay

>90% (HPLC)

form

powder

solubility

H2O: ~10 mg/mL

shipped in

wet ice

storage temp.

−20°C

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Application

Pyoverdines from Pseudomonas fluorescens has been used :
  • in tryptophan fluorescence quenching to determine its binding to neutrophil-gelatinase-associated lipocalin (NGAL)
  • to detect pyoverdine diffusion surrounding siderophore-conjugated monobactam (MB-1)-resistant colonies in chelexed, dialyzed Mueller-Hinton broth (CDMHB)
  • for synchronous fluorescence spectra of purified pyoverdine

Biochem/physiol Actions

Pyoverdines help in promoting plant growth by chelating iron and rendering it unavailable for plant pathogens. The ferri-pyoverdine complex acts as a signaling molecule inducing the production of secreted virulence factors in Pseudomonas aeruginosa.
Pyoverdines, also called pseuobactins and pyoverdins, are fluorescent siderophores that have high affinity for iron (1032 M-1), and are synthesized by fluorescent pseudomonads under iron-deficient growth conditions. Pyoverdines were shown to prevent iron toxicity produced by iron overload in hepatocyte cultures and effectively scavenges the hydroxyl and peroxyl radicals. Pyoverdines are effective in acquiring iron from transferrin and lactoferrin. Pyoverdines are also involved in the suppression of pythium-induced damping-off of tomato and promotion of growth in some higher plants.

Physical form

Supplied as a mixture containing mainly the succinic acid (MW=1161), 2-hydroxy glutaramide (MW=1190), and succinamide (MW=1160) forms of pyoverdines.

related product

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Adaptation-based resistance to siderophore-conjugated antibacterial agents by Pseudomonas aeruginosa.
Tomaras AP
Antimicrobial Agents and Chemotherapy, 57(9), 4197-4207 (2013)
Pyoverdine, the Major Siderophore in Pseudomonas aeruginosa, Evades NGAL Recognition.
Peek ME
Interdisciplinary perspectives on infectious diseases, 843509-843509 (2012)
Correction: Multicolor Whole-Cell Bacterial Sensing Using a Synchronous Fluorescence Spectroscopy-Based Approach.
PLoS ONE (2015)
Purification of Pyoverdines of Pseudomonas fluorescens 2-79 by Copper-Chelate Chromatography.
Xiao R and Kisaalita WS
Applied and Environmental Microbiology, 61(11), 3769-3774 (1995)
Biological activity of secondary metabolites produced by a strain of Pseudomonas fluorescens.
Boruah HP and Kumar BS
Folia Microbiologica, 47(4), 359-363 (2002)

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