L0401
Lectin from Lycopersicon esculentum (tomato)
FITC conjugate, buffered aqueous solution
Synonym(s):
Lycopersicon esculentum agglutinin, LEA
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
buffered aqueous solution
Pricing and availability is not currently available.
conjugate
FITC conjugate
Quality Segment
form
buffered aqueous solution
extent of labeling
2.0-6.0 mol FITC per mol lectin
storage temp.
2-8°C
General description
Lectins are carbohydrate binding proteins. Lectin from Lycopersicon esculentum (LEA) binds strongly to polylactosamine oligosaccharides. It is the major component of endothelial and epithelial cell surface carbohydrate.
Application
Lectin from Lycopersicon esculentum (tomato) has been used:
- for immunofluorescence staining
- for the quantification of vascular perfusion
- for the evaluation of gel perfusion efficiency
Biochem/physiol Actions
Lectin has anti-cancer, anti-human immunodeficiency virus (anti-HIV), anti-microbial infection and blocks mucosal atrophy. It reduces type 2 diabetes and obesity, stimulates nutrient absorption and drug targeting. It is used to stain the vascular tree in the spinal cord and brain of animals. Lectin agglutinates cells and precipitates polysaccharides and glycoproteins.
LEA is not blood group specific, but has an affinity for N-acetyl-β-D-glucosamine oligomers. The lectin is a glycoprotein containing approx. equal amounts of protein and carbohydrate and is reported to inhibit the mitogenic activity of phytohemagglutinin from Phaseolus vulgaris. This lectin binds endothelial cells and is used to visualize the microvasculature.
Packaging
Sold on the basis of mg active conjugate.
Physical form
Solution in 10 mM HEPES, 0.15 M NaCl, pH 7.5, containing 0.1 mM Ca2+, 0.08% sodium azide and 5 mg/mL β-cyclodextrin.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.3, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25 °C.
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This Item | |||
|---|---|---|---|
| form buffered aqueous solution | form saline suspension | form saline suspension | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| conjugate FITC conjugate | conjugate agarose conjugate | conjugate Sepharose™ conjugate | conjugate biotin conjugate |
| extent of labeling 2.0-6.0 mol FITC per mol lectin | extent of labeling 5-10 mg per mL | extent of labeling ~2 mg per mL | extent of labeling 2-4 mol biotin per mol protein |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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