L6512

Lectin from Helix pomatia

biotin conjugate, lyophilized powder

• Synonym(s): Helix pomatia agglutinin, HPA
• NACRES: NA.32
• UNSPSC Code: 12352202
• Form: lyophilized powder

Properties

• conjugate: biotin conjugate
• Quality Segment: 200
• form: lyophilized powder
• extent of labeling: 2-4 mol biotin per mol protein
• impurities: salt, free
• storage temp.: −20°C

Description

General description

Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. These oligomeric proteins are characterized by the presence of a carbohydrate recognition domain.

Application

Lectin from Helix pomatia has been used to study its binding activities in Gyrodactylus derjavini parasitizing fins of Oncorhynchus mykiss.

Biochem/physiol Actions

HPA has anti-A human blood group specificity and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Lectin is useful in glycoconjugate characterizing, imaging and targeting. Lectin participates in host recognition and tissue adhesion. Lectins shows resistance to proteolytic degradation in vivo.

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)