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A375-C5 Cell Line human

97011320, human skin, Epithelial-like

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About This Item

UNSPSC Code:
41106514

product name

A375-C5 Cell Line human, 97011320

biological source

human skin

description

Human malignant melanoma IL-1 insensitive

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human malignant melanoma IL-1 insensitive

Cell Line Description

The cell line A375-C5 is unresponsive to the anti-proliferative effect of IL-1. It has been derived from the parental line A373 (Sigma catalogue no. 88113005) which was subjected to cloning by limiting dilution. This method resulted in the establishment of A375-C5 and an IL-1 sensitive clone, A375-C6 (Sigma catalogue no. 97011321). The unresponsiveness of A375-C5 is probably due to an IL-1 receptor deficiency. The cells have been eradicated from mycoplasma at ECACC.

Application

IL-1 resistance studies, receptor studies

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% FBS

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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