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product name
CCO, 95060212
biological source
fish ovary
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Channel Catfish ovary
Cell Line Description
CCO was established from the ovaries of a healthy, juvenile channel catfish, Ictalurus punctatus. The cells grow at temperatures from 10°C to 35°C with the optimum at 30°C and with a reported population doubling up to 12 hours. CCO cells have been shown to replicated channel catfish virus but were refractory to 12 other viruses from fish, birds and mammals.
Application
Isolation of channel catfish virus
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 30°C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media ch
Other Notes
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Certificates of Analysis (COA)
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