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TGP 52

94022429, mouse pancreas, Trabecular

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About This Item

UNSPSC Code:
41106514

product name

TGP 52, 94022429

biological source

mouse pancreas

growth mode

Adherent

karyotype

Not specified

morphology

Trabecular

products

Somatostatin

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse islet cell tumour

Cell Line Description

TGP 52 has been isolated from an islet tumour arising in the pancreas of a transgenic Tg (E1a-1-SV40E) Bri18 mouse. These mice carry a pseudogene construct composed of a pancreatic specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 52 cells are SV40T antigen positive and secrete somatostatin, it is one of a series of pancreatic tumour lines deposited at ECACC. Cells transiently produce insulin in culture followed by somatostatin.

Application

Study of endocrine pancreas carcinomas, hormone assays.

Culture Medium

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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