KELLY Cell Line human
92110411, human brain, Neuronal
Synonym(s):
NB19 Cells, NB19-RIKEN Cells
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About This Item
UNSPSC Code:
41106514
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product name
KELLY Cell Line human, 92110411, neuroblastoma
biological source
human brain
growth mode
Adherent
karyotype
Not specified
morphology
Neuronal
products
N-myc RNA or protein
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human neuroblastoma
Cell Line Description
The cells possess a genomic amplification of the N-myc gene and express elevated levels of N-myc RNA or protein.
Application
KELLY cell line has been used to study the role of polysialic acid (PSA) in controlling tumor cell growth and differentiation by interfering with neural cell adhesion molecule (NCAM) signaling. It has also been used to study the role of protein tyrosine phosphatase SHP1 in inhibiting Ret oncogene activity.
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
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