KARPAS 422, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., from human lymph(lymphoma), 06101702
biological source
human lymph (lymphoma)
packaging
tube of 5 μg 06101702-DNA-5UG pkg of vial of cells 06101702-1VL
growth mode
Suspension
karyotype
Hyperdiploid with 10% polyploidy
morphology
Round polygonal cells growing singly or in small clusters in suspension
Established from a pleural effusion of 73 year-old woman diagnosed with B-cell non-Hodgkin lymphoma (intra-abdominal, diffuse large cell lymphoma, refractory, terminal). Carries t(14;18) IGH-BCL2 fusion gene (break point in major breakpoint region, MBR).
Maintain cultures between 0.5 - 2 x 1000,000 cells/ml; 5% CO2; 37 °C. Doubling time approximately 60-90 hours. Split saturated culture 1:2 every 2-4 days. Round polygonal cells growing singly or in small clusters in suspension.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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