A9883

ADA

≥98% (titration)

• Synonym(s): N-(2-Acetamido)iminodiacetic acid, N-(Carbamoylmethyl)iminodiacetic acid
• Linear Formula: H₂NCOCH₂N(CH₂CO₂H)₂
• CAS Number: 26239-55-4
• Molecular Weight: 190.15
• Beilstein: 1787181
• EC Number: 247-530-0
• MDL number: MFCD00008031
• UNSPSC Code: 12161700
• PubChem Substance ID: 24891404
• NACRES: NA.25

Properties

• Quality Level: 400
• Assay: ≥98% (titration)
• form: powder
• pH: 1.5-3.0
• useful pH range: 6.0-7.2
• pKa (25 °C): 6.6
• mp: 219 °C (dec.) (lit.)
• solubility: 1 M NaOH: 160 mg/mL, clear, colorless
• application(s): diagnostic assay manufacturing
• storage temp.: room temp
• SMILES string: NC(=O)CN(CC(O)=O)CC(O)=O
• InChI: 1S/C6H10N2O5/c7-4(9)1-8(2-5(10)11)3-6(12)13/h1-3H2,(H2,7,9)(H,10,11)(H,12,13)
• InChI key: QZTKDVCDBIDYMD-UHFFFAOYSA-N

Description

General description

ADA is a zwitterionic buffer used in biochemistry and molecular biology. It is one of the Good buffers developed in the 1960′s to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The pioneering publication by Good and co-workers describes the synthesis of ADA and its physical properties. The useful range of ADA buffer in aqueous solution is 6.0 - 7.2.

Application

Biological buffer component with sodium hydroxide and sodium chloride (pH 5.67-7.57, useful pH range 6.4-7.4). Used to prepare immobilized pH gradients.

Preparation Note

This product is soluble in 1 M NaOH (160mg/mL), yielding a clear, colorless solution.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302
• Precautionary Statements: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves