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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
6G8, monoclonal
mol wt
calculated mol wt 48.77 kDa
species reactivity
human
packaging
antibody small pack of 100 μg
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1κ
UniProt accession no.
shipped in
dry ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... GLA(2717)
General description
a-Galactosidase A (UniProt: P06280; EC:3.2.1.22, Alpha-D-galactosidase A, Alpha-D-galactoside galactohydrolase, Galactosylgalactosylglucosylceramidas GLA, Melibiase, Agalsidase) is encoded by the GLA gene (Gene ID: 2717) in human. a-galactosidase A is a homodimeric, lysosomal enzyme that catalyzes the hydrolysis of terminal alpha-galactosyl moieties from glycolipids and glycoproteins and participates to their degradation in the lysosome. It is synthesized with a signal peptide (aa 1-31), which is subsequently cleaved off to generate the mature form that is heavily glycosylated with asparagine-linked high mannose groups. Mutations in GLA gene have been linked to Fabry disease that is characterized by glycolipid accumulation in various tissues and leads to other complication such as renal failure and cardiac and cerebral complications.
Specificity
Clone 6G8 is a mouse monoclonal antibody that specifically detects human a-Galactosidase A.
Immunogen
Recombinant full-length human a-Galactosidase A (without the signal peptide).
Application
Quality Control Testing
Evaluated by Immunocytochemistry in Acetone fixed HEK 293 cells..
Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected a-Galactosidase A in acetone fixed HEK 293 cells.
Tested Applications
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected a-Galactosidase A in HeLa cells.
Immunohistochemistry Applications: A representative lot detected a-Galactosidase A in Immunohistochemistry applications (Tian, W., et. al. (2019). Nat Commun. 10(1):1785).
Immunocytochemistry Analysis: A representative lot detected a-Galactosidase A in acetone fixed HEK293 cells either HEK293 overexpressing GLA (A), HEK293 wild type (WT; B), or HEK293 cells with KO of GLA (C) (Courtesy of Asst. Prof. Zhang Yang, Ph.D., and Assoc. Prof. Ulla Mandel, Ph.D., Copenhagen Center for Glycomics, University of Copenhagen, Denmark).
Western Blotting Analysis: A representative lot detected a-Galactosidase A in lysates from HEK293 cells overexpressing GLA, HEK293 (WT), but not in HEK293 cells with GLA knockout (Courtesy of Asst. Prof. Zhang Yang, Ph.D., and Assoc. Prof. Ulla Mandel, Ph.D., Copenhagen Center for Glycomics, University of Copenhagen, Denmark).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Immunocytochemistry in Acetone fixed HEK 293 cells..
Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected a-Galactosidase A in acetone fixed HEK 293 cells.
Tested Applications
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected a-Galactosidase A in HeLa cells.
Immunohistochemistry Applications: A representative lot detected a-Galactosidase A in Immunohistochemistry applications (Tian, W., et. al. (2019). Nat Commun. 10(1):1785).
Immunocytochemistry Analysis: A representative lot detected a-Galactosidase A in acetone fixed HEK293 cells either HEK293 overexpressing GLA (A), HEK293 wild type (WT; B), or HEK293 cells with KO of GLA (C) (Courtesy of Asst. Prof. Zhang Yang, Ph.D., and Assoc. Prof. Ulla Mandel, Ph.D., Copenhagen Center for Glycomics, University of Copenhagen, Denmark).
Western Blotting Analysis: A representative lot detected a-Galactosidase A in lysates from HEK293 cells overexpressing GLA, HEK293 (WT), but not in HEK293 cells with GLA knockout (Courtesy of Asst. Prof. Zhang Yang, Ph.D., and Assoc. Prof. Ulla Mandel, Ph.D., Copenhagen Center for Glycomics, University of Copenhagen, Denmark).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-a-galactosidase A, clone 6G8, Cat. No. MABS1935, is a mouse monoclonal antibody that detects -a-galactosidase A and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at +2°C to +8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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