400012

Caspase-1 Inhibitor II

The Caspase-1 Inhibitor II, also referenced under CAS 178603-78-6, controls the biological activity of Caspase-1. This small molecule/inhibitor is primarily used for Cancer applications.

• Synonym(s): Caspase-1 Inhibitor II, IL-1β Converting Enzyme (ICE) Inhibitor II, Ac-YVAD-CMK
• Empirical Formula (Hill Notation): C₂₄H₃₃ClN₄O₈
• CAS Number: 178603-78-6
• Molecular Weight: 540.99
• UNSPSC Code: 12352200
• NACRES: NA.54
• MDL number: MFCD00237124
• Assay: ≥95% (HPLC)
• Form: solid
• Storage condition: OK to freeze, desiccated

Properties

• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated
• color: white
• solubility: DMSO: 5 mg/mL
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: ClCC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)C)Cc1ccc(cc1)O)C(C)C)C)CC(=O)O
• InChI: 1S/C24H33ClN4O8/c1-12(2)21(24(37)26-13(3)22(35)28-17(10-20(33)34)19(32)11-25)29-23(36)18(27-14(4)30)9-15-5-7-16(31)8-6-15/h5-8,12-13,17-18,21,31H,9-11H2,1-4H3,(H,26,37)(H,27,30)(H,28,35)(H,29,36)(H,33,34)/t13-,17-,18-,21-/m0/s1
• InChI key: UOUBHJRCKHLGFB-DGJUNBOTSA-N

Description

General description

A cell-permeable and irreversible inhibitor of caspase-1 (K_i = 760 pM), caspase-4, and caspase-5. Inhibits Fas-mediated apoptosis and acidic sphingomyelinase activation.

Interleukin-1β converting enzyme (ICE) is a cysteine protease that cleaves the IL-1β precursor to mature IL-1β. Caspase-1 inhibitor II is a cell-permeable, irreversible inhibitor of Caspase-1, Caspase-4, and Caspase-5. Inhibits CPP32/Apopain at a much higher concentration. Inhibits Fas-mediated apoptosis and acidic sphingomyelinase activation.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
caspase-1

Product does not compete with ATP.

Reversible: no

Target K_i: 760 pM against caspase-1

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Ac-Tyr-Val-Ala-Asp-CMK

Brenner, B., et al. 1998. Cell Death Differ. 5, 29.
Garcia-Calvo, M., et al. 1998. J. Biol. Chem. 273, 32608.
Hilbi, H., et al. 1998. J. Biol. Chem. 273, 32895.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Schlegel, J., et al. 1996. J. Biol. Chem.271, 1841.
Enari, M., et al. 1995. Nature375, 78.
Walker, N.P.C., et al. 1994. Cell78, 343.
Thornberry, N., et al. 1992. Nature356, 768.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable