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Interferon-γ Protein, Recombinant human

Name: Interferon-γ Protein, Recombinant human
Brand: MM

The Interferon-g protein (or IFN-gamma protein) is a lymphoid factor which possesses potent anti-viral activity.

Synonym(s):

Human Interferon, Human Interferon-γ, Recombinant Interferon-γ

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.77

eCl@ss:

32160405

Assay:

>98% (SDS-PAGE and HPLC)

Biological source:

human

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Properties

biological source

human

Quality Segment

100

assay

>98% (SDS-PAGE and HPLC)

specific activity

≥2 x 10(E7) U/mg

manufacturer/tradename

Chemicon^®

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 ng/μg endotoxin (of IFN-gamma; 1EU/μg)

input

sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)

NCBI accession no.

NM_000619.2

UniProt accession no.

P01579

shipped in

dry ice

storage temp.

-10 to -25°C

Gene Information

human ... IFNG(3458)

Description

General description

Interferon-g (IFN-gamma) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Human IFN-gamma is a 16.7 kDa protein containing 143 amino acid residues.

Product Source: Human, expressed in E.coli

Application

For most in vitro applications, IFN-gamma exerts its biological activity in the concentration range of 0.1 to 10 ng/mL. Responding cells are (partial list): Lymphocytes.

Physical form

Sterile filtered, lyophilized from 10 mM sodium phosphate, pH 7.4.

Preparation Note

The lyophilized IFN-g, though stable at room temperature, is best stored desiccated at -20°C. After a quick spin, reconstitute in 1x PBS, pH 8.0 to a concentration of 0.5-1.0 mg/mL. Note: Further dilutions should be made into RPMI + 10% FCS or 5% HSA and stored at 4°C for 1 week or -20°C for future use. Reconstituted material should be stored in undiluted aliquots at -20°C for up to six months.

Analysis Note

Specific Activity: Human IFN-gamma is fully biologically active when compared to standards.The ED(50) as determined in a viral resistance assay is ≤ 0.05 ng/mL

Other Notes

Replaces: IF3

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item	SRP3058	IF005	GF305
Sigma-Aldrich IF002 Interferon-γ Protein, Recombinant human	Sigma-Aldrich SRP3058 IFN-γ human	Sigma-Aldrich IF005 Interferon-γ Protein, Recombinant mouse	Sigma-Aldrich GF305 IFN- γ Protein, Human Recombinant Animal Free
description The Interferon-g protein (or IFN-gamma protein) is a lymphoid factor which possesses potent anti-viral activity.	description Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), suitable for cell culture	description The Interferon-gamma protein (or IFN-gamma protein) is a regulatory protein produced by activated NK cells & CD4+TCRalpha/beta+, CD8+TCRalpha/beta+ & TCRgamma/delta+ T cells.	description The Interferon gamma (or IFN-γ protein) is an acid-labile interferon produced by CD4 & CD8 T lymphocytes as well as activated NK cells. Manufactured using all non-animal reagents.
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) -
biological source human	biological source human	biological source mouse	biological source -
assay >98% (SDS-PAGE and HPLC)	assay ≥98% (SDS-PAGE)	assay >98% (SDS-PAGE and HPLC)	assay >98% (SDS-PAGE and HPLC)
shipped in dry ice	shipped in wet ice	shipped in dry ice	shipped in dry ice
impurities <0.1 ng/μg endotoxin (of IFN-gamma; 1EU/μg)	impurities <0.1 EU/μg endotoxin, tested	impurities <0.1 ng/μg endotoxin (of IFN-gamma; 1EU/μg)	impurities <0.1 μg/mg protein endotoxin (<1EU/μg)
storage temp. -10 to -25°C	storage temp. −20°C	storage temp. -10 to -25°C	storage temp. -10 to -25°C

Storage Class

11 - Combustible Solids

wgk

WGK 3

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