IF005

Interferon-γ Protein, Recombinant mouse

The Interferon-gamma protein (or IFN-gamma protein) is a regulatory protein produced by activated NK cells & CD4+TCRalpha/beta+, CD8+TCRalpha/beta+ & TCRgamma/delta+ T cells.

• Synonym(s): IFN γ, IFN g, IFN gamma, INF γ, INF g, INF gamma
• UNSPSC Code: 12352202
• NACRES: NA.77
• eCl@ss: 32160405
• Form: liquid
• Assay: >98% (SDS-PAGE and HPLC)
• Biological source: mouse

Properties

• biological source: mouse
• Quality Segment: 100
• assay: >98% (SDS-PAGE and HPLC)
• form: liquid
• specific activity: ≥1.0 x 10E7 U/mg
• manufacturer/tradename: Chemicon^®
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 ng/μg endotoxin (of IFN-gamma; 1EU/μg)
• input: sample type mesenchymal stem cell(s); sample type hematopoietic stem cell(s)
• UniProt accession no.: P01579, P01580
• shipped in: dry ice
• storage temp.: -10 to -25°C

Description

General description

Interferon-gamma (IFN-gamma) is a regulatory protein produced by activated NK cells and CD4+TCRalpha/beta+, CD8+TCRalpha/beta+, and TCRgamma/delta+ T cells. The biological activities of IFN-gamma include the specific binding to a single class of high affinity receptors. In addition, IFN-gamma can upgrade the number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. Recombinant Murine IFN-γ is a 134 amino-acid length protein with a predicted molecular weight of 15.6 kDa. C-terminal truncation due to proteolytic cleavage occurs during E. coli fermentation and results in a 15.1 kDa protein confirmed by mass spectroscopy. SDS-PAGE analysis of Recombinant Murine IFN-γ shows a band that migrates at approximately 11-12 kDa, under nonreducing and reducing conditions.

Product Source: Mouse, expressed in E.coli

Application

For most in vitro applications, IFN-gamma exerts its biological activity in the concentration range of 0.1 to 10.0 ng/mL.

Biochem/physiol Actions

The ED50 as determined by a cytopathic effect inhibition assay with mouse NIH 3T3 cells challenged VSV is ≤ 0.1 ng/mL

Physical form

Sterile filtered, lyophilized from 10mM Sodium Phosphate, pH 7.0 + 25mM NaCl.

Preparation Note

The lyophilized protein is stable for a few weeks at room temperature, but is best stored at -20°C. After a quick spin, reconstitute with Sodium Phosphate, pH 8.0 to a concentration of 1 mg/mL. Further dilutions should be made into RPMI + 10% FCS or 5% HAS and stored at 2-8ºC for one week or -20ºC for future use. Reconstituted IFN-gamma should be stored in undiluted aliquots at -20°C for up to six months from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2