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525200-M

Sigma-Aldrich

Phospholipase D, Streptomyces chromofuscus

Synonym(s):

Phospholipase D, Streptomyces chromofuscus, PLD

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About This Item

CAS Number:
9001-87-0
Enzyme Commission number:
3.1.4.4 (BRENDA, IUBMB)
MDL number:
MFCD00131897
UNSPSC Code:
12352202
NACRES:
NA.54

form

lyophilized solid

Quality Level

100

specific activity

≥50 units/mg dry wt.

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

color

brownish

solubility

10 mM Tris-HCl, pH 8.0, 0.05% BSA, and 0.1% (w/v) TRITON™ X-100: 1 mg/mL

foreign activity

Aatalase, glucose oxidase, none detected

storage temp.

−20°C

General description

Native phospholipase D from Streptomyces chromofuscus. Catalyzes the hydrolysis of lecithin to phosphatidic acid and choline. Coupled with choline oxidase, it can also be used for the determination of phospholipids in serum.
Native phospholipase D from Streptomyces chromofuscus. Catalyzes the hydrolysis of lecithin to phosphatidic acid and choline; coupled with choline oxidase, it can be used for the determination of phospholipids in serum.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of phosphatidylcholine per min at 37°C, pH 8.0.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 6 months at 4°C or for up to 1 year at -20°C.

Other Notes

Kurz, T., et al. 1993. Circ. Res. 72, 701.
Natarajan, V., and Garcia, J.G. 1993. J. Lab. Clin. Med. 121, 337.
Yamamoto, I., et al. 1993. Biochim. Biophys. Acta1145, 293.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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