Upon the addition of crystalline serum albumin (10 mg/mL) and storage at -15°C, solutions of this product in water are stable for several months. Additional information can be found in the Methods in Enzymology XIV, 202 (1969).
P8398
Phospholipase D from cabbage
Type IV, lyophilized powder, ≥100 units/mg solid
Synonym(s):
Lecithinase D, Phosphatidylcholine phosphatidohydrolase, Phospholipase D from white cabbage
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-639-8
MDL number:
Specific activity:
≥100 units/mg solid
Pricing and availability is not currently available.
type
Type IV
form
lyophilized powder
specific activity
≥100 units/mg solid
composition
Protein, ~25%
storage temp.
−20°C
General description
Application
Phospholipase D (PLD) is used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation[2]. Furthermore, PLD is used in purification and kinetic studies[3].
The enzyme has been used for the preparation of Bodipy-phosphatidylcholine during the preparation of fluorescently labelled lipids.[4]
Biochem/physiol Actions
Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Phospholipase D from cabbage is widely used in biocatalytic transphosphatidylation reactions.[5]
The amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD[6]. It hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Analysis Note
Protein determined using biuret.
Other Notes
One unit will liberate 1.0 μmol of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥100 units/mg solid | specific activity ≥150 units/mg solid | specific activity ≥1,000 units/mg protein (Lowry) | specific activity 10-50 units/mg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| composition Protein, ~25% | composition - | composition - | composition protein, 20-40% |
| type Type IV | type Type VII | type - | type Type I |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
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