402176-M
ImmunoPen
This ImmunoPen is validated for use in Frozen Sections, Immunostaining Barrier, Paraffin Sections.
Synonym(s):
pen for immunostaining
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About This Item
UNSPSC Code:
41116133
NACRES:
NA.32
Recommended Products
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
storage temp.
10-30°C
General description
Designed to minimize antibody waste in immunostaining techniques on paraffin sections, frozen sections, or cytology preparations. The pen contains liquid resistant to water, alcohol, and acetone. When applied in a circle around the specimen the liquid forms a hydrophobic barrier, retaining antibody and minimizing waste of valuable reagents. Multiple sections, separated by individ-ual circular barriers, can be applied to the same slide. The ImmunoPen can be used for immunostaining procedures employing the following methods: PAP (peroxidase-antiperoxidase), ABC, enzymatic, and frozen section. While the barrier is insoluble in alcohol and acetone, it can be removed by xylene after the staining procedure is complete.
Designed to minimize waste of antibody in immunostaining techniques on paraffin sections, frozen sections, or cytology preparations. When applied in a circle around the specimen, forms a hydrophobic barrier, retaining antibody. Sufficient for 500-800 individual applications. Not available for sale in Japan.
Application
Frozen Sections
Immunostaining Barrier
Paraffin Sections
Immunostaining Barrier
Paraffin Sections
Warning
Toxicity: Standard Handling (A)
Reconstitution
Keep cap tightly closed when pen is not in use, and store horizontally. Lasts for ~500-800 applications.
Other Notes
To start the ImmunoPen shake well and gently press the tip straight down several times until the fluid starts soaking the tip.
Paraffin Section Method:
Deparaffinize and rehydrate the section according to normal procedures. For best adhesion, dry around the section with tissue paper and encircle it with the ImmunoPen. Allow the circle to dry for approximately 1-2 min at room temperature, and then return the slide to water or buffer. If trypsinization or other proteolytic digestion is required, apply the ImmunoPen barrier after the digestion is completed.
Frozen Section Method:
Apply the ImmunoPen circle after cleaning slides with xylene/ethanol or coating with egg albumin, glycerin, or poly-L-lysine. Apply before fixation or first immersion in water or buffer. If the slide is treated with a hydrophile, soak it in 0.1N hydrochloric acid for 20 s, then wash with water.
Paraffin Section Method:
Deparaffinize and rehydrate the section according to normal procedures. For best adhesion, dry around the section with tissue paper and encircle it with the ImmunoPen. Allow the circle to dry for approximately 1-2 min at room temperature, and then return the slide to water or buffer. If trypsinization or other proteolytic digestion is required, apply the ImmunoPen barrier after the digestion is completed.
Frozen Section Method:
Apply the ImmunoPen circle after cleaning slides with xylene/ethanol or coating with egg albumin, glycerin, or poly-L-lysine. Apply before fixation or first immersion in water or buffer. If the slide is treated with a hydrophile, soak it in 0.1N hydrochloric acid for 20 s, then wash with water.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Ozone 1 - Repr. 1B - Skin Irrit. 2 - STOT RE 2 Inhalation - STOT SE 3
Target Organs
Central nervous system, Liver,Central nervous system, Respiratory system
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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