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208670

Sigma-Aldrich

Calmodulin, His•Tag® Human, Recombinant

Calmodulin, His•Tag Human, Recombinant, is a full-length, recombinant human calmodulin fused to a His•Tag sequence at the N-terminus. Contains four functional Ca2+-binding sites.

Synonym(s):

Calmodulin, Human, Recombinant

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

Pricing and availability is not currently available.

biological source

human

Quality Level

Assay

>90% (SDS-PAGE)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

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This Item
SRP6104SRP6105SRP6370
assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

biological source

human

biological source

human

biological source

bacterial

biological source

human

technique(s)

activity assay: suitable

technique(s)

-

technique(s)

activity assay: suitable

technique(s)

-

form

liquid

form

liquid

form

liquid

form

liquid

mol wt

37.4 kDa (342 aa, 1-334 aa + CT His Tag)

mol wt

35.2 kDa (335 aa, 25-338 aa + NT His Tag)

mol wt

34.9 kDa (336 aa, 1-312 aa + NT His Tag)

mol wt

41.5 kDa (384 aa, 1-364 aa + His Tag)

General description

Full-length, recombinant human calmodulin fused to a HisTag sequence at the N-terminus. Contains four functional Ca2+-binding sites (aa 20-31; aa 56-67; aa 93-104; aa 129-140) with EF-hands (aa 7-42; aa 43-78; aa 80-115; aa 116-148); and a ubiquitination site at Lys21. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Through its interaction with Ca2+, calmodulin mediates the control of a large number of enzymes, including protein kinases and phosphatases. It is expected to be phosphorylated at Thr44 by CaMK4. In addition, it serves as a substrate for various protein tyrosine kinases.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 0.2% Protease Inhibitor Cocktail Set VII (Cat. No. 539138).

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Chou, J.J., et al. 2001.Nat. Struct. Biol.8, 990.
Lennon, G., et al. 1996.Genomics33, 151.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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