LC/MS Analysis of Lactic Acid Enantiomers in Urine on Astec® CHIROBIOTIC® R
Materiales
analytical column
mobile phase component
CONDITIONS
column
Astec CHIROBIOTIC R, 15 cm x 2.1 mm I.D., 5 μm particles (13019AST)
mobile phase
[A] 33.3 mM ammonium acetate; [B] acetonitrile; (15:85, A:B)
flow rate
0.7 mL/min
column temp.
4 °C
detector
(-)ESI
injection
20 μL
sample
Urine extracts in 5% (v/v) acetic acid, 10% (v/v) H2O and 85% (v/v) acetonitrile
Descripción
Analysis Note
Chromatogram supplied courtesy of H. Henry and associates at the Clinical Chemistry Laboratory, Centre Hospitalier Universitaire Vaudois, University of Lausanne, CH-1011, Switzerland. View the related full-length contributed article in Reporter 30.1.
Other Notes
Reference: Biomed. Chromatogr. 2011 "Sensitive determination of D-lactic acid and L-lactic acid in urine by high-performance liquid chromatography–tandem mass spectrometry" H. Henry, N. Marmy Conus, P. Steenhout, A. Béguin and O. Boulat, Published online in Wiley Online Library, wileyonlinelibrary.com/journal/bmc.
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC