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814007

TSKgel® Phenyl-5PW RP (10 µm) HPLC Columns

L x I.D. 7.5 cm × 8 mm, phenyl phase, octadecyl

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About This Item

UNSPSC Code:
41115711

product name

TSKgel® Phenyl-5PW RP HPLC Column, phase phenyl, octadecyl, L × I.D. 7.5 cm × 8 mm, 10 μm particle size

material

glass

product line

TSKgel®

manufacturer/tradename

Tosoh 14007

extent of labeling

1 meq/mL ligand density (estimate)

parameter

no limitation salt concentration
0-100% organic solvent
2.5 mL/min flow rate
20 bar max. pressure
4-60 °C temperature

technique(s)

HPLC: suitable

L × I.D.

7.5 cm × 8 mm

matrix

hydroxylated methacrylic polymer

matrix active group

phenyl phase, octadecyl

particle size

10 μm

operating pH

2-12

separation technique

reversed phase

General description

Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of strong basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel® columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
  • Octadecyl-2PW particles have 125 A pores
  • Octadecyl-4PW particles have 500 Apores
  • Phenyl-5PW RP particles have 1000 A pores
  • Octadecyl-NPR particles are nonporous

Physical form

Shipped in 30% methanol - 70% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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