The guard column kit for Product No. 565325-U is 565371-U. This kit includes one cartridge, stand-alone holder, a piece of tubing, 2 nuts and 2 ferrules. A set of two replacement cartridges is Product No. 565370-U.
565325-U
Ascentis® RP-Amide (5 µm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
Sinónimos:
RP-Amide Reversed-Phase Chromatography Column
About This Item
Productos recomendados
Nombre del producto
Ascentis® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Materiales
stainless steel column
Agency
suitable for USP L60
Línea del producto
Ascentis®
Características
endcapped
fabricante / nombre comercial
Ascentis®
envase
1 ea of
Extensión del etiquetado
19.5% Carbon loading
Parámetros
≤70 °C temp. range
400 bar pressure (5801 psi)
técnicas
HPLC: suitable
LC/MS: suitable
L × D.I.
25 cm × 4.6 mm
superficie
450 m2/g
cobertura de la superficie
2.7 μmol/m2
impurezas
<5 ppm metals
Matriz
fully porous particle
silica gel high purity, spherical
grupo activo de la matriz
amide, alkyl phase
tamaño de partícula
5 μm
tamaño de poro
100 Å
Intervalo de pH operativo
2-8
aplicaciones
environmental
food and beverages
técnica de separación
reversed phase
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Descripción general
Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.
Aplicación
- A validated UHPLC method for the determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts using an RP-Amide fused-core column.: This study presents a validated Ultra-High-Performance Liquid Chromatography (UHPLC) method employing an RP-Amide fused-core column for the quantitative determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. The method demonstrated excellent precision, accuracy, and sensitivity, making it a valuable tool for the analysis of these bioactive compounds in complex matrices. The research highlights the utility of the Ascentis® RP-Amide HPLC Column in enhancing the resolution and efficiency of chromatographic separations in analytical chemistry applications (Fibigr et al., 2018).
Características y beneficios
- Excellent retention and peak shape for polar compounds
- 100% aqueous compatibility
- Ultra low bleed, LC-MS compatible
- Unique selectivity
Información legal
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cartucho de precolumna
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Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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What is the Product No of the guard column for Product No. 565325-U Ascentis RP-Amide HPLC column?
1 answer-
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What solvent should Product No. 565325-U Ascentis RP-Amide HPLC Column be stored in?
1 answer-
Product No. 565325-U should be stored in 100% acetonitrile after any buffers have been flushed out.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can I use Ascentis Express on a UHPLC system?
1 answer-
Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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