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HybridSPE®-Phospholipid solid phase extraction (SPE) Cartridge

Cartridge, bed wt. 30 mg, volume 1 mL, pk of 100

Sinónimos:

HybridSPE (phospholipid and protein removal) SPE cartridge tube, 1 mL

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About This Item

UNSPSC Code:
41115712
NACRES:
NB.21

product name

HybridSPE®-Phospholipid, Cartridge, bed wt. 30 mg, volume 1 mL, pk of 100

material

PE frit (20 μm)
polypropylene hardware

product line

HybridSPE®

composition

bed wt., 30 mg

packaging

pk of 100

technique(s)

solid phase extraction (SPE): suitable

volume

1 mL

matrix active group

zirconia-based phase

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General description

HybridSPE-Phospholipid technology is a simple and generic sample prep platform designed for the gross level removal of endogenous protein and phospholipid interferences from biological plasma and serum prior to LC-MS or LC-MS/MS analysis. Biological plasma or serum is first subjected to protein precipitation via the addition and mixing of acidified acetonitrile. Precipitated proteins are then removed by centrifugation and the resulting supernatant is loaded on the HybridSPE-Phospholipid 96-well plate or cartridge which acts as a chemical filter that specifically targets the removal of endogenous sample phospholipids. The phospholipid retention mechanism is based on a highly selective Lewis acid-base interaction between the proprietary zirconia ions functionally bonded to the HybridSPE-Phospholipid stationary phase and the phosphate moiety consistent with all phospholipids. The resulting eluent is ready for immediate LC-MS or LC-MS-MS analysis.

The "In-well" and "In-cartridge" precipitation methods are available for the HybridSPE-Phospholipid 96-well version and HybridSPE-Phospholipid Ultra cartridge in which biological plasma/serum is first added to either the well or cartridge, followed by acidified acetonitrile (precipitation agent). After a brief mixing/vortexing step, vacuum is applied. Because the 96-well and Ultra cartridge versions contain a series of low porosity hydrophobic filters/frits, the packed-bed filter/frit assembly acts as a depth filter facilitating the concurrent removal of both phospholipids and precipitated proteins during the extraction process. Standard HybridSPE-Phospholipid cartridges require an "off-line" precipitation method.

Application


  • Less is more: a methodological assessment of extraction techniques for per- and polyfluoroalkyl substances (PFAS) analysis in mammalian tissues.: This study assesses various extraction methods, including HybridSPE®-Phospholipid, for analyzing PFAS in tissues, highlighting efficiency and reproducibility (Mertens et al., 2023).

  • Rapid analysis of 65 pharmaceuticals and 7 personal care products in plasma and whole-body tissue samples of fish using acidic extraction, zirconia-coated silica cleanup, and liquid chromatography-tandem mass spectrometry.: This paper describes a rapid analytical method incorporating HybridSPE®-Phospholipid for sample preparation, demonstrating its application in environmental toxicology (Tanoue et al., 2020).


Features and Benefits

  • For use in conjunction with off-line protein precipitation.
  • Merges the simplicity of protein precipitation and the selectivity of SPE via the targeted removal of phospholipids
  • Reduce ion-suppression through the complete removal of phospholipids and precipitated proteins
  • 2-3 step generic procedure
  • Minimal to no method development
  • Available in 96-well and 1 mL cartridge dimensions

Legal Information

HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

Referencia del producto
Descripción
Precios

related product

Referencia del producto
Descripción
Precios

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificados de análisis (COA)

Lot/Batch Number
18931401
18686101
18640301
18500901
18265401

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Artículos

Vitamin D epi-Metabolites Separated with HybridSPE-Phospholipid

This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.

Ion-Suppression & Phospholipid Contamination

An article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.

HybridSPE-Phospholipid Technology

This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.

Protocolos

Enrichment of Phospholipids in Biological Samples Using HybridSPE-PL

A simple method to enrich phospholipids from plasma samples, involving a HybridSPE-PPT 96-well plate that both retains phospholipids and removes precipitated proteins.

Contenido relacionado

HPLC de moléculas pequeñas

Las moléculas pequeñas son iones y compuestos de peso molecular normalmente inferior a 900 dalton. Estos compuestos pueden separarse y analizarse con eficacia mediante HPLC, UHPLC y LC-MS utilizando principalmente partículas de sílice o fases estacionarias monolíticas con una amplia gama de composiciones químicas de la columna (modificaciones).

Small Molecule HPLC

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

Chromatograms

application for HPLC

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