T9691

Anti-phospho-TrkA (pTyr⁴⁹⁰) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

• MDL number: MFCD01092507
• UNSPSC Code: 51111800
• NACRES: NA.41

Propiedades

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• mol wt: antigen 140 kDa
• species reactivity: rat, mouse, human
• technique(s): immunocytochemistry: 1:100 using cultured cells; immunohistochemistry (frozen sections): 1:10; immunoprecipitation (IP): 1:250; western blot: 1:1,000 using NGF-treated PC12 cells
• UniProt accession no.: P04629
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pTyr490)
• Gene Information: human ... NTRK1(4914) ; mouse ... Ntrk1(18211) ; rat ... Ntrk1(59109)

Descripción

General description

TrkA belongs to the Trk proto-oncogene family and is expressed mainly in central and peripheral nervous systems. When TrkA binds to NGF, it autophosphorylates tyrosine residues like-Tyr⁴⁹⁰, Tyr⁶⁷⁴, Tyr⁶⁷⁵, Tyr⁷⁵¹, and Tyr⁷⁸⁵ and subsequently activates many downstream effector proteins. Defects in TrkA lead to congenital insensitivity to pain with anhidrosis. Anti-phospho-TrkA (pTyr⁴⁹⁰) antibody can be used in immunocytochemistry (1:100, using cultured cells). It is also useful in identifying endogenous levels of phosphorylated Tyr⁴⁹⁰ in TrkA, TrkB and TrkC. Rabbit anti- phospho-TrkA (pTyr⁴⁹⁰) antibody is reactive in mouse, human and rat.

Immunogen

synthetic phosphopeptide Tyr⁴⁹⁰ corresponding to residues surrounding Tyr⁴⁹⁰ of human TrkA, conjugated to KLH. This sequence is highly conserved in TrkB and TrkC.

Application

Anti-phospho-TrkA (pTyr⁴⁹⁰) antibody can be used in immunoprecipitation, immunoblotting and immunohistochemistry. It may also be used in western blotting when diluted to ratio of 1:1000 for NGF-treated PC12 cells.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, and 150 mM sodium chloride containing 100 μg/ml bovine serum albumin and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable