The sequence of this product is not mapped.
T0706
Tropoelastin human
recombinant, expressed in E. coli, 1 mg
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1 MG
US$ 631,00
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1 MG
US$ 631,00
About This Item
US$ 631,00
PRECIOS SIN IMPUESTOS NACIONALES
Check Cart for Availability
Productos recomendados
recombinante
expressed in E. coli
Nivel de calidad
descripción
1 mg recombinant human Tropoelastin as lyophilized powder.
esterilidad
electron beam irradiated
concentración
1 mg
impurezas
≤10 EU/mL Endotoxin
temp. de almacenamiento
−20°C
Descripción general
Tropoelastin is a 60-72 kDa alternatively spliced extracellular matrix protein.[1] It is a soluble precursor of elastin and is distributed in elastic tissues.[2][3] Tropoelastin consists of an N- terminal 26-amino-acid signal peptide and highly basic strongly conserved C-terminal domain, that is required for both incorporation into elastin and for cellular interactions. It Is also characterized with a hydrophobic domain and exon 26A, which is an unusual highly hydrophilic domain.[2][1]
Aplicación
Preparation Procedure
Use this procedure as a guideline to determine optimal coating conditions for the culture system of choice.
1. Add 1 ml of sterile 0.25% glacial acetic acid solution to the tropoelastin serum vial containing 1 mg.
2. Mix contents gently until material is completely solubilized. The solution may remain slightly hazy.
3. Transfer desired volume of solution from the serum vial to a dilution vessel if required. Further dilute to desired concentration using sterile 0.25% Acetic Acid solution. A typical working concentration may range from 1 to 50 μg/ml.
4. Add appropriate amount of diluted tropoelastin material to the culture surface.
5. Incubate at room temperature or 37 °C, covered, for approximately 1.5 hours.
6. After incubation, aspirate any remaining material.
7. Rinse coated surfaces carefully with sterile water and avoid scratching surfaces.
8. Coated surfaces are ready for use. They may also be stored at 2-10 °C damp or air dried if sterility is maintained.
Note: To maintain sterility, perform all operations in a laminar flow hood employing aseptic techniques.
Use this procedure as a guideline to determine optimal coating conditions for the culture system of choice.
1. Add 1 ml of sterile 0.25% glacial acetic acid solution to the tropoelastin serum vial containing 1 mg.
2. Mix contents gently until material is completely solubilized. The solution may remain slightly hazy.
3. Transfer desired volume of solution from the serum vial to a dilution vessel if required. Further dilute to desired concentration using sterile 0.25% Acetic Acid solution. A typical working concentration may range from 1 to 50 μg/ml.
4. Add appropriate amount of diluted tropoelastin material to the culture surface.
5. Incubate at room temperature or 37 °C, covered, for approximately 1.5 hours.
6. After incubation, aspirate any remaining material.
7. Rinse coated surfaces carefully with sterile water and avoid scratching surfaces.
8. Coated surfaces are ready for use. They may also be stored at 2-10 °C damp or air dried if sterility is maintained.
Note: To maintain sterility, perform all operations in a laminar flow hood employing aseptic techniques.
Acciones bioquímicas o fisiológicas
Características y beneficios
Tropoelastin available in its native state. Unique since it becomes cross-linked immediately after its synthesis by the cell and during its export into the extracellular matrix.
Calidad
Identity and purity qualitatively determined by SDS-PAGE
Otras notas
Storage at -20 °C prior to solubilization is recommended. Storage at 2-10 °C after reconstitution is recommended.
Shelf life is 24 months when stored at -20 °C. Shelf life after reconstitution is 3 months when stored at 2-10 °C
Shelf life is 24 months when stored at -20 °C. Shelf life after reconstitution is 3 months when stored at 2-10 °C
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Certificados de análisis (COA)
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Nasim Annabi et al.
Advanced materials (Deerfield Beach, Fla.), 28(1), 40-49 (2015-11-10)
A highly elastic hybrid hydrogel of methacryloyl-substituted recombinant human tropoelastin (MeTro) and graphene oxide (GO) nanoparticles are developed. The synergistic effect of these two materials significantly enhances both ultimate strain (250%), reversible rotation (9700°), and the fracture energy (38.8 ±
Biochemistry of tropoelastin.
Vrhovski B and Weiss A S
European Journal of Biochemistry, 258(1), 1-18 (1998)
Coacervation of tropoelastin results in fiber formation.
Cox B A, et al.
The Journal of Biological Chemistry, 249(3), 997-998 (1974)
Tropoelastin.
Wise S G and Weiss A S
The International Journal of Biochemistry & Cell Biology, 41(3), 494-497 (2009)
Jeff Holst et al.
Nature biotechnology, 28(10), 1123-1128 (2010-10-05)
Surprisingly little is known about the effects of the physical microenvironment on hemopoietic stem and progenitor cells. To explore the physical effects of matrix elasticity on well-characterized primitive hemopoietic cells, we made use of a uniquely elastic biomaterial, tropoelastin. Culturing
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Hi, Can you send me the sequence of this protein? do you have any peptide mapping or data confirming the sequence?
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