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RAB0610

Sigma-Aldrich

Human APOB / Apolipoprotein B-100 ELISA Kit

Sinónimos:

Apo B-100, Apolipoprotein B-100

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2.5 ng/mL
standard curve range: 2.458-600 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... APOB(338)

General description

The ApoB ELISA kit provides for the Quantitative measurement of ApoB-100 in Cell Culture Supernatants, Plasma and Serum.
The APOB (apolipoprotein B) gene is mapped to human chromosome 2p24.1.[1] Apob protein is an amphipathic protein that exists as α- helices and β-sheets. Apob is present in the major lipoproteins : chylomicrons, low density lipoproteins and very low density lipoprotein.[2]

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Apob (apolipoprotein B) functions in the low-density lipoprotein cholesterol segregation. Increased APOB gene expression is observed in non-alcoholic fatty liver disease and contributes to its pathogenesis.[3] Reduced Apob protein is indicated in familial hypobetalipoproteinemia.[4]

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion
GHS05

signalword

Warning

hcodes

H290

Hazard Classifications

Met. Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0214K0095
1129J0095
0624I0095
0420I0095
0225I0095

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Identification of novel APOB mutations by targeted next-generation sequencing for the molecular diagnosis of familial hypobetalipoproteinemia.
Rimbert A, et al.
Atherosclerosis, 250, 52-56 (2016)
Lipoproteomics: using mass spectrometry-based proteomics to explore the assembly, structure, and function of lipoproteins.
Hoofnagle A N and Heinecke J W
Journal of Lipid Research, 50(10), 1967-1975 (2009)
Serum apoB levels independently predict the development of non-alcoholic fatty liver disease: A 7-year prospective study.
Wang J, et al.
Liver International : Official Journal of the International Association For the Study of the Liver, 37(8), 1202-1208 (2017)
Alejandro Escobedo et al.
Nutrients, 13(11) (2021-11-28)
Snack alternatives based on common beans (Phaseolus vulgaris L.) have been developed to promote pulse consumption. The purpose of this study was to evaluate the chemical composition, sensory acceptance and the effect of common bean baked snack (CBBS) consumption on
Forty-three loci associated with plasma lipoprotein size, concentration, and cholesterol content in genome-wide analysis.
Chasman DI, et al.
PLoS Genetics, 5(11), e1000730-e1000730 (2009)

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